Nano therapeutic agent and preparation method and application thereof

A therapeutic agent and nanotechnology, applied in the field of biomedical nanomaterials, can solve the problem of low therapeutic effect

Active Publication Date: 2019-11-05
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a nano-therapeutic agent and its preparation method and application, aiming to solve the problem of low therapeutic effect of existing hydrophobic chemotherapy drugs and glucose oxidase

Method used

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  • Nano therapeutic agent and preparation method and application thereof
  • Nano therapeutic agent and preparation method and application thereof
  • Nano therapeutic agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Synthesis of Sorafenib-glucose oxidase nano-therapeutic agent (SRF@GOx)

[0050]Add 20 mg of glucose oxidase (GOx) to 10 mL of phosphate buffered saline (PBS, pH 7.4) so ​​that the concentration of glucose oxidase in the obtained mixture is 2 mg / mL, stir and add β-mercaptoethanol, and then slowly add 600 μL of 5 mg / mL of sorafenib (SRF, soluble in ethanol), after the dropwise addition, the above mixture was centrifuged at 6000rpm for 5min, and the precipitate was collected for redissolution to obtain sorafenib-glucose oxidase nanoparticles.

[0051] TEM image of the synthesized sorafenib-glucose oxidase nanotherapeutic agent (SRF@GOx) figure 1 shown. from figure 1 It can be seen that the synthesized sorafenib-glucose oxidase nano-therapeutic agent is in the shape of a sphere, and the particle size of the nano-therapeutic agent is between 100nm and 150nm.

Embodiment 2

[0052] Example 2: Analysis of Zeta potential changes before and after synthesis of Sorafenib-glucose oxidase nanotherapeutic agent (SRF@GOx)

[0053] Zeta potential changes of sorafenib, glucose oxidase and sorafenib-glucose oxidase nano-therapeutic agent were detected by Zeta potential meter.

[0054] Zeta potential diagram of the synthesized sorafenib-glucose oxidase nanotherapeutic agent (SRF@GOx) figure 2 shown. from figure 2 It can be seen that Sorafenib is positively charged (about 5.5mV), and glucose oxidase is negatively charged (about-23.2mV), and the potential value of the synthesized Sorafenib-glucose oxidase nano-therapeutic agent is about-8.7mV, It shows that the formed nanoparticles are the product of combination of Sorafenib and glucose oxidase.

Embodiment 3

[0055] Example 3: Identification of Effects of Different Treatments on the Secondary Structure of Glucose Oxidase

[0056] The effect of different treatments on the secondary structure of glucose oxidase was evaluated by circular dichroism spectrometer. The secondary structures of simple glucose oxidase, sorafenib-glucose oxidase nano-therapeutic agent and the enzyme in its release solution were determined respectively. The result is as image 3 Shown, the Sorafenib-glucose oxidase nano-therapeutic agent that forms can not detect the secondary structure of glucose oxidase substantially, but, in the supernatant after Sorafenib-glucose oxidase nano-therapeutic agent releases, can It was detected that the glucose oxidase secondary structure was restored.

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Abstract

The invention discloses a nano therapeutic agent and a preparation method and an application thereof. The nano therapeutic agent includes glucose oxidase and a hydrophobic chemotherapy drug located ina hydrophobic cavity of the glucose oxidase. The nano therapeutic agent is a compound of glucose oxidase and the hydrophobic chemotherapy drugs, wherein the glucose oxidase and the hydrophobic chemotherapy drugs are combined by hydrophobic effect; and the nano therapeutic agent can realize targeted delivery of tumor, chemotherapy and hunger-like combined therapy at the same time.

Description

technical field [0001] The invention relates to the field of biomedical nanomaterials, in particular to a nano therapeutic agent and its preparation method and application. Background technique [0002] The starvation-like treatment of tumors is a new treatment method, which mainly cuts off the blood and nutrient supply of tumor cells to starve them to death, so as to achieve a certain therapeutic effect. At present, a large number of studies have proved that this therapy can effectively inhibit the rapid growth of tumors, and has little side effects on the body, so it has attracted more and more attention from scientists in recent years. Among them, glucose oxidase (GOx) has attracted great attention in the context of cancer therapy over the past few years due to its inherent biocompatibility and biodegradability and its unique catalytic properties towards β-d-glucose. research interests. GOx can effectively catalyze the oxidation of glucose to gluconic acid and hydrogen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K45/06A61K38/44A61K31/44A61P35/00
CPCA61K9/5169A61K31/44A61K38/443A61K45/06A61P35/00C12Y101/03004A61K2300/00
Inventor 黄鹏江珊珊张一帆林静
Owner SHENZHEN UNIV
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