Expandable ultra-filtration centrifugation pipe using AAO porous membrane

Abstract

The invention discloses an expandable ultra-filtration centrifugation pipe using an AAO porous membrane. The expandable ultra-filtration centrifugation pipe comprises a cover, a filtrate cylinder, a plurality of filtering layers and a bottom cylinder, wherein the cover, the filtrate cylinder, the plurality of the filtering layers and the bottom cylinder are sequentially connected, the filtering layer comprises a sealing ring, an AAO porous membrane, a support sheet and a cascade member, the sealing ring, the AAO porous membrane, the support sheet and the cascade member are sequentially and closely attached, the filtrate cylinder is closely attached to the seal ring in the filtering layer adjacent to the filtrate cylinder, the cascade member of the filtering layer is closely attached to theseal ring in the filtering layer adjacent to the cascade member, and the bottom cylinder is connected to the cascade member in the filtering layer adjacent to the bottom cylinder. According to the present invention, by utilizing the AAO porous membrane as the ultra-filtration membrane, the pore size is uniform, and the filtration effect is precise and reliable; the filtering layers can be expanded, such that the layer-to-layer separation of particles having different particle sizes can be achieved, multi-stage filtration can be simultaneously achieved, and the particles having a certain medium particle size can be precisely obtained; and the expandable ultra-filtration centrifugation pipe has advantages of simple structure and convenient installation, wherein various components are tightly connected so as to effectively prevent the filtrate leakage.

Description

technical field [0001] The invention relates to the technical field of ultrafiltration centrifuge tube preparation, in particular to an expandable ultrafiltration centrifuge tube using an AAO (anodized aluminum oxide) porous membrane. Background technique [0002] Centrifugal technology has been widely used in biological sciences, especially in the fields of biochemistry and molecular biology research. Centrifugal technology is mainly used for the separation and preparation of various biological samples. The suspension of biological samples is placed in a centrifuge tube under high-speed rotation. Due to the huge centrifugal force, the suspended tiny particles (such as cell organelles, sedimentation of biological macromolecules, etc.) ) settles at a certain speed, thereby being separated from the solution. Centrifuge tubes are important equipment in centrifugation technology, generally tubular containers with lids. The ultrafiltration centrifuge tube is to install an ultra...

AI Technical Summary

Problems solved by technology

At present, there are four main types of ultrafiltration membranes in ultrafiltration centrifuge tubes, namely polyethersulfone, cellulose triacetate, regenerated cellulose, and Hydrosart (a modified regenerated cellulose). Due to the relatively low uniformity of the pore size, particles of different particle sizes cannot be effectively separated during the ultrafiltration centrifugation process, resulting in a low retention rate of nanoparticles, and it is difficult to achieve accurate separation of particles

[0003] In addition, in the field of fine filtration, samples are often mixed with nanoparticles of multiple sizes (such as 50 nm, 100 nm, 200 nm), and some applications require high-precision separation of particles in a certain intermediate size range, with a single ultrafiltration Membrane ultrafiltration centrifuge tubes cannot meet such needs at one time and need to be centrifuged multiple times. However, due to the poor uniformity of the ultrafiltration membrane pore size in traditional ultrafiltration centrifuge tubes, the filtration effect is poor, and even multiple centrifugations are difficult. Achieve high-precision separation

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