Straight-line scanning-type PCR instrument and method for PCR detecting using straight line scanning type PCR instrument

Abstract

The invention relates to a straight-line scanning-type PCR instrument for PCR chip detecting. The straight-line scanning-type PCR instrument comprises a temperature control assembly, a detecting partand a control mechanism; the temperature control assembly comprises more than two chip temperature control modules, each chip temperature control module comprises a chip loading assembly with functions of solely-heating and temperature control, and each chip loading assembly loads a PCR reaction chip and controls the temperature of the PCR reaction chip; the detecting part comprises an optical detecting element and a scanning motion mechanism, and the optical detecting element is fixedly arranged on the scanning motion mechanism moving along a straight line; and the optical detecting element comprises an excitation light source and a detecting device, and the detecting device sequentially collects and detects all the PCR reaction chips. The straight-line scanning-type PCR instrument is used for detecting PCR chips, new detection can be started when a round of PCR is conducted by the instrument, and the situation that detection can be conducted along with arriving can be truly achieved.

Description

technical field [0001] The invention relates to the fields of biology and analytical chemistry, in particular to a linear scanning PCR instrument for PCR chip detection and a PCR detection method using the same. Background technique [0002] PCR (polymerase chain reaction) is an important means of molecular biology research, the reaction time has always been the limiting factor of PCR reaction, a conventional PCR reaction often takes one or two hours, in clinical testing, especially for emergency, so The long detection time limits its application field. With the development and application of PCR fast reagents, the bottleneck of reaction time is mainly concentrated on the heating and cooling speed. Based on this, how to improve the heating and cooling speed of PCR reaction has become the key to its expanded application. In addition, in the past, PCR testing equipment often started a new round of testing after the end of one round. In emergency applications, the demand for o...

AI Technical Summary

Problems solved by technology

Most of the current PCR instruments put the samples into the sample tubes and put them into the instrument at the same time. After all the tests are completed, the results will be obtained. On the one hand, such PCR instruments cannot be checked at any time. They must wait for all the previous samples to be completed before the next test. In experiments, on the other hand, sample tubes (such as 8-row tube caps), due to their tubular structure, heat up and cool down slowly, which is not conducive to improving the efficiency of PCR instrument amplification.

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