Simple environment-friendly tissue RNA extraction device

Abstract

The invention relates to a simple and environment-friendly tissue RNA extraction device. The device comprises a bottom plate, a protective cover, a grinding mechanism, a cleaning mechanism and a liquid feeding mechanism, the protective cover is arranged on the bottom plate, the grinding mechanism is arranged on one side of the top of the protective cover, the cleaning mechanism is arranged on thelower part of the grinding mechanism and on the bottom plate, and the liquid feeding mechanism is arranged on the other side of the top of the protective cover. The device has the advantages of scientific and reasonable design, simple and convenient structure, simple operation and flexible movement, effectively saves operation time and space, reduces labor cost, electrically adds liquid, accurately adjusts the adding amount of a reagent, reduces the contact frequency of operators and harmful reagents, delivers scientific research personnel from complicated experimental operation, effectively reduces the harm of toxic experimental reagents to human bodies, and ensures the personal safety of experimental operators. The device reduces artificial errors, simultaneously solves the problem of reagent cross pollution caused by repeated liquid addition, carries out the whole operation process in a low-temperature environment, effectively avoids the RNA degradation, and ensures that high-quality RNA is extracted.

Description

technical field [0001] The invention belongs to the field of molecular biology and cytology experiment devices, and mainly relates to the extraction of RNA, in particular to a simple and environment-friendly tissue RNA extraction device. Background technique [0002] PCR technology is widely used in the study of gene expression due to its specificity, sensitivity, high efficiency, rapidity, and good repeatability. Western blot technology is widely used in the detection of protein expression levels. The two techniques often need to be separated from tissues and cells. and purified RNA. The principle of RNA extraction is to crush cells or tissues with denaturants, then extract RNA with organic solvents such as chloroform, and then precipitate, wash, dry, and finally dissolve, mainly through crushing tissues, separating RNA, precipitating RNA, washing RNA, and melting RNA, preserve RNA. [0003] The existing traditional RNA extraction method is mainly the Trizol method, which...

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Problems solved by technology

[0004] In RNA extraction, the degradation of RNA is one of the common problems. Since ribonuclease (RNase) is ubiquitous, experimental operators' hand sweat, saliva, etc., dust, pipette tips with RNase, EP tubes, etc. Exogenous RNase may degrade RNA at any time, a small amount of RNase will cause RNA degradation during preparation and analysis, and the purity and integrity of the prepared RNA can directly affect the results of RNA analysis, so The preparation and analysis of RNA is extremely difficult, and a slight negligence will lead to the failure of the experiment

In addition, the reagents used in the prior art are highly toxic, and if the grinding or sample addition is not timely during batch operations, the RNA in the sample will be degraded, affecting the purity and concentration of the collected RNA.

[0005] Through the search of existing published patent documents, no published patent documents identical or similar to this patent application were found

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