Isochrysis zhanjiangensis culture medium and large-scale culture method thereof in floating type photobioreactor

A technology of photobioreactor and Isochrysis, which is applied in the field of microalgae cultivation, can solve the problems of uncertain cultivation process, etc., and achieve the effect of cultivating algae in original flavor, avoiding the loss of microalgae nutrition, and increasing profits

Active Publication Date: 2019-11-08
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While the concentration of bicarbonate is an important factor affecting the growth of microalgae, the cultivation process of this aspect is currently uncertain, especially at the large-scale cultivation level.

Method used

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  • Isochrysis zhanjiangensis culture medium and large-scale culture method thereof in floating type photobioreactor
  • Isochrysis zhanjiangensis culture medium and large-scale culture method thereof in floating type photobioreactor
  • Isochrysis zhanjiangensis culture medium and large-scale culture method thereof in floating type photobioreactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 contains the influence of the culture medium of different concentrations of sodium bicarbonate on the growth of Isochrysis zhanjiang

[0041] First utilize filtered seawater to prepare the Isochrysis Zhanjiang culture medium without carbon source of the present invention, the composition and final concentration of its formula are: NaNO 3 0.375g / L, NaH 2 PO 4 2H 2 O 0.0325g / L, Na 2 EDTA0.0218g / L, VB120.0025g / L, VB1 0.5g / L, VH 0.0025g / L, f / 2 trace elements 1ml / L; among them, f / 2 trace elements are prepared from sterile distilled water, and its composition The points are as follows: CuSO 4 ·5H 2 O 0.01g / L, FeCl 3 ·6H 2 O 3.15g / L, ZnSO 4 ·7H 2 O0.022g / L, CoCl 2 ·6H 2 O 0.01g / L, MnCl 2 4H 2 O 0.18g / L, Na 2 MoO 4 2H 2 O 0.006g / L. Then, based on this, sodium bicarbonate was added according to the final concentration of 1.0, 3.0, 7.0 and 9.0g / L, so as to obtain Zhanjiang et al. Chrysopsis full nutrient medium.

[0042] Cultivation method: the me...

Embodiment 2

[0045] The influence of embodiment 2 different carbon sources on the growth of Isochrysis Zhanjiang

[0046] As described in Example 1, the present invention determines that the concentration of sodium bicarbonate is the most suitable for the growth of Isochrysis Zhanjiang. In the traditional culture method, air and 2% CO are mainly used. 2 air as a carbon source to cultivate Isoflagellates zhanjiang. Therefore, present embodiment selects preferred 5g L -1 The concentration of sodium bicarbonate was used as the research object, and the concentration of cultured cells and the volumetric yield were used as the indicators to investigate and compare the effects of these three carbon sources on the growth of Isoflagellates Zhanjiang.

[0047] First prepare Zhanjiang Isochrysis Zhanjiang culture medium A, B, C (recipe sees embodiment 1) of the present invention not containing carbon source, then add sodium bicarbonate in B, obtain the Zhanjiang etc. that final concentration is 5.0...

Embodiment 3

[0050] The influence of embodiment 3 liquid layer thickness on the growth of Isochrysis Zhanjiang

[0051]In the floating photobioreactor culture system, the thickness of the liquid layer not only affects the average light intensity entering the culture liquid, but also has an important impact on the movement of the reactor and the mixing of the internal fluid, thus affecting the growth efficiency of microalgae. Therefore, this embodiment uses a 1.0 square meter floating photobioreactor and a preferred 5.0g L -1 The concentration of sodium bicarbonate was used as a research tool, and the concentration of cultured cells and volumetric yield were used as indicators to investigate the effects of three liquid layer thicknesses of 5.0, 7.5 and 10 cm on the growth of Isochrysis Zhanjiang.

[0052] First prepare the Zhanjiang Isochrysis Zhanjiang full nutrient medium (recipe sees embodiment 1) containing 5.0g / L sodium bicarbonate of the present invention, and inoculate the algal cell...

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Abstract

The invention discloses an isochrysis zhanjiangensis culture medium and a large-scale culture method thereof in a floating type photobioreactor. The isochrysis zhanjiangensis culture medium is prepared from seawater or filtered seawater and comprises components as follows: 1.0-9.0 g / L of NaHCO3, 0.375 g / L of NaNO3, 0.0325 g / L of NaH2PO4.2H2O, 0.0218 g / L of Na2EDTA, 0.0025 g / L of VB12, 0.5 g / L of VB1, 0.0025 g / L of VH and 1 ml / L of f / 2 microelements. The method for culturing the isochrysis zhanjiangensis on a large scale by the floating type photobioreactor can realize efficient and stable production of isochrysis zhanjiangensis so as to provide a large quantity of isochrysis zhanjiangensis biomass timely for shell culture enterprises and aquaculture related enterprises and guarantee timelysupply of microalgae organism bait. More importantly, compared with a traditional photobioreactor production method, the method has lower culture cost, so that the profits of aquaculture enterprisesare increased.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a Zhanjiang Isoflagellate culture medium and a cultivation method. Background technique [0002] Isochrysis zhangjiangensis belongs to Chrysohyta, Prymnesiophyceae, Isochrysidales, Isochrysidaceae, Isochrysis ). The strain of algae has high photosynthetic efficiency and fast growth speed, can be cultivated by using seawater, brackish water and municipal sewage, and is easy to cultivate on a large scale. More importantly, the algal cells of this strain are rich in nutrients and rich in unsaturated fatty acids, such as DHA and EPA. In addition, because it has no cell wall, it is easily ingested and digested by marine animals, so it is considered to be an ideal bait for artificial breeding of marine animals. In addition, it is also used as a high-quality open feed for mussels, oysters, cockles, prawns and other valuable aquatic products. It is the key material ba...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 迟占有朱陈霸韩德森
Owner DALIAN UNIV OF TECH
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