Application of polyethylene glycol to dyeing animal tissue section grease

An animal tissue and polyethylene glycol technology, which is used in the preparation, sampling, and measuring devices of test samples, can solve the problems that the slices cannot be stored for a long time, the slices are easily folded, and the quality of the slices is affected. Complete, avoid shrinkage deformation, avoid the effect of displacement

Inactive Publication Date: 2019-11-08
武汉赛维尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the staining process, due to the section being too thick, it is easy to fold when staining and attaching the section, which affects the quality of the section
Thermal expansion and contraction will cause lipid droplets to gather together, making the fat cells "vacuolated", and the staining results will be negative, and the slices cannot be stored for a long time, so timely observation is required, and there are certain limitations

Method used

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  • Application of polyethylene glycol to dyeing animal tissue section grease
  • Application of polyethylene glycol to dyeing animal tissue section grease
  • Application of polyethylene glycol to dyeing animal tissue section grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of method utilizing polyethylene glycol to carry out the method for fat staining of mouse liver animal tissue section, comprises the steps:

[0034] (1) Take a normal healthy mouse liver tissue sample, about 0.5cm×0.5cm×0.5cm, fix it with fixative solution, wash it with running water, and absorb the water with absorbent paper;

[0035] (2) Place the mouse liver tissue sample in carbowax I (polyethylene glycol with a molecular weight of 1000), and soak it in wax for 60 minutes in an incubator at 60°C;

[0036] (3) Place the mouse liver tissue sample in carbowax II (mixture of carbowax with a molecular weight of 1500 and a molecular weight of 1000 at a ratio of 1:2), and immerse it in a 60°C incubator for 60 minutes;

[0037] (4) Place the mouse liver tissue sample in carbowax II (mixture of carbowax with molecular weight of 1500 and molecular weight of 1000 at a ratio of 1:1), and soak in wax for 60 minutes in an incubator at 60°C;

[0038] (5) Place the mouse li...

Embodiment 2

[0043] A kind of method utilizing polyethylene glycol to carry out fatty liver mouse liver slice oil staining method, comprises the steps:

[0044] (1) Immediately after fatty liver model mice were sacrificed under anesthesia, liver tissue samples were taken, about 0.5cm×0.5cm×0.5cm, fixed with fixative, rinsed with running water, and dried with absorbent paper;

[0045] (2) Place mouse fatty liver in carbowax I (polyethylene glycol with a molecular weight of 1000), and soak in wax for 90 minutes in an incubator at 55°C;

[0046] (3) Put mouse fatty liver in carbowax II (1:2 mixture of carbowax with molecular weight of 1500 and molecular weight of 1000), and soak in wax for 120 minutes in an incubator at 55°C;

[0047] (4) Put the mouse fatty liver in carbowax II (mixture of carbowax with a molecular weight of 1500 and a molecular weight of 1000 at a ratio of 2:1), and soak it in a 55°C incubator for 120 minutes;

[0048] (5) Put the mouse fatty liver in carbowax II (mixture ...

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Abstract

The invention belongs to the technical field of pathological tissue section making, and particularly relates to application of polyethylene glycol to dyeing animal tissue section grease. Through the excellent water solubility characteristic of the polyethylene glycol, with the polyethylene glycol as the dehydrating agent, the permeation and embedding formation of a medium are realized by means ofembedding in the dehydrating process, a formed tissue embedding block has the appearance characteristic of a paraffin block, the embedded medium can be removed by washing the tissue embedding block without processing the tissue embedding block with an organic solvent, and the problem that grease loss is caused by organic solvent processing is avoided. Tissue sections prepared by the method have the thickness of 3-5 micron, and the problem that tissue overlapping is caused by the too high thickness of the frozen sections is avoided. After the tissue sections prepared by the method are dyed by oil red O, due to the dehydration and permeation of the polyethylene glycol, most of grease drops are completely preserved in cytoplasm, the problem of displacement of grease drops in the frozen sections is avoided, and the problem that a tissue structure is not complete since ice crystals are generated in the frozen sections at a low temperature is also avoided.

Description

technical field [0001] The invention belongs to the technical field of making pathological tissue slices, and in particular relates to the application of polyethylene glycol for oil staining of animal tissue slices. Background technique [0002] Lipid is a general term for neutral fats, lipids and their derivatives, mainly referring to neutral fats. Under normal circumstances, except for fat cells, there are generally no or only a small amount of lipid droplets in other cells. When lesions occur, lipid droplets or lipid droplets will increase significantly in the cells, especially when fatty degeneration occurs in solid organs such as the heart, liver, and kidney, vacuoles of different sizes will appear in the cytoplasm, and fat staining is required to identify vacuoles. Whether the blisters belong to steatosis or watery degeneration or glycogen storage. In addition, in atherosclerosis, the lipid deposition under the endothelial cells can be displayed with fat staining. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/06
CPCG01N1/06G01N1/286G01N1/30G01N2001/2873
Inventor 黄静张高英田巧云
Owner 武汉赛维尔生物科技有限公司
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