Regional stem cell-induced active decellularized bone-tendon interface book-page scaffold

A technology of inducing activity and stem cells, which is applied in the field of bone and tendon interface injury repair, can solve the problems of ineffective promotion of rapid and high-quality healing of patients, difficulty in achieving rapid and high-quality healing, and patients' inability to resume sports as soon as possible, so as to avoid explosive release, avoid cell loss, and improve biological activity

Active Publication Date: 2022-03-29
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for these multi-phase scaffolds to simulate the dual bionics of the bone-tendon interface morphology and mechanical properties of the organism at the same time, so that patients cannot resume sports as soon as possible, and it is difficult to achieve rapid and high-quality healing; some bionic scaffold grafts need a certain period of time in the body. The process of chemical absorption, and the loading of stem cells cannot provide strong intrinsic induction activity, making it difficult for the damaged tissue / organ to achieve the most suitable regenerative microenvironment, and cannot effectively promote the rapid and high-quality healing of patients

Method used

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  • Regional stem cell-induced active decellularized bone-tendon interface book-page scaffold

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Experimental program
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Effect test

Embodiment 1

[0028] refer to figure 1 , regional stem cell-induced active decellularized bone-tendon interface page scaffold, the specific preparation steps of the scaffold are:

[0029] Step 1. Acquisition of osteogenic bioactive factors, chondrogenic bioactive factors and tendon bioactive factors;

[0030] Specifically: obtain hBMP-2, hTGF-β3 and hGDF-7, and prepare 100ng / ml working solution of hBMP-2, hTGF-β3 and hGDF-7;

[0031] The hBMP-2, hTGF-β3 and hGDF-7 can be purchased as commercialized human-derived biologically active factor products, such as Human BMP-2 (PeproTech96-120-02-100), Human TGF-beta3 (PeproTech 96-AF-100-36E-250), Human GDF-7 (PeproTech 96-120-37-100), further prepared 100ng / ml hBMP-2, hTGF-β3 and hGDF-7 working solutions.

[0032] Step 2. Preparation of hBMP-2, hTGF-β3 and hGDF-7 slow-release colostrum:

[0033] Take 20mg of polylactic acid-glycolic acid copolymer, add 1mL of dichloromethane, fully shake to dissolve PLGA completely; take 100ng / ml hBMP-2, 100ng...

Embodiment 2

[0043] The regional stem cell-induced activity of the present application, the decellularized bone-tendon interface leaf scaffold and the scaffold of the prior art were used for the same degree of bone-tendon interface injury, and it was found that the scaffold of the present application was less damaged than the scaffold of the prior art The patients recovered quickly and healed with high quality.

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Abstract

The invention belongs to the technical field of bone-tendon interface injury repair, and specifically relates to regional stem cell-induced activity decellularized bone-tendon interface leaf support, specifically comprising: step 1, obtaining hBMP-2, hTGF-β3 and hGDF-7, and preparing 100ng / ml working solution of hBMP-2, hTGF-β3 and hGDF-7; Step 2, preparation of hBMP-2, hTGF-β3 and hGDF-7 slow-release colostrum; Step 3, preparation of double bionic decellularized bone tendon interface leaf scaffold ; Step 4, preparation of regional directional induced slow-release decellularized bone tendon interface leaf scaffold; step 5, preparation of regional stem cell-induced activity decellularized bone tendon interface leaf scaffold; providing a natural and natural shape for clinical bone tendon interface reconstruction A new type of tissue engineering graft with reliable mechanics and excellent activity can achieve rapid and high-quality healing after bone-tendon interface injury.

Description

technical field [0001] The invention belongs to the technical field of repairing bone-tendon interface damage, and in particular relates to a decellularized bone-tendon interface leaf support induced by regional stem cells. Background technique [0002] With the vigorous development of national sports and fitness activities, the national physical fitness and health awareness have been steadily improved, but it is inevitable that the incidence of various sports injuries has also increased. Among them, bone-tendon interface injuries (such as cruciate ligament injuries, rotator cuff injuries) are the most common type of sports injuries. According to statistics, in the United States alone, about 250,000 rotator cuff injuries and 100,000 anterior cruciate ligament injuries require surgical treatment each year. Although my country currently lacks relevant epidemiological data, compared with the data in the United States, bone-tendon interface injury has become an urgent challenge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L27/40A61L27/38A61L27/36A61L27/18A61L27/22A61L27/50A61L27/54
CPCA61L27/3834A61L27/3847A61L27/3852A61L27/386A61L27/3895A61L27/3687A61L27/3691A61L27/3604A61L27/365A61L27/3654A61L27/3662A61L27/18A61L27/227A61L27/50A61L27/54A61L2430/40A61L2300/602A61L2300/252A61L2430/10A61L2430/06A61L2430/02A61L2300/412
Inventor 吕红斌陈灿陈洋胡建中
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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