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Dendrobium huoshanense polysaccharide with anti-digestive system cancer effect

A technology of Dendrobium Huoshanense and digestive system, which is applied in the field of polysaccharides of Chinese herbal medicine, can solve the problems of lack of polysaccharides with anticancer activity, unclear chemical structure of polysaccharides with anticancer activity of Dendrobium Huoshanense, and restrictions on the research and development of new anticancer drug polysaccharides of Dendrobium Huoshanense, so as to achieve good therapeutic digestion Systemic cancer effect, environmental friendliness, and structural stability effect

Active Publication Date: 2019-11-15
HEFEI UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been reports involving the structural identification of the polysaccharides of Dendrobium Huoshanense, almost all polysaccharides related to anti-cancer are extracted from the cultures or stems of Dendrobium Huoshanense. structure, and the lack of a suitable extraction and purification process to prepare anticancer active polysaccharides with clear chemical structures and uniform components, all of which limit the research and development of new anticancer drug polysaccharides from Dendrobium huoshanense

Method used

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  • Dendrobium huoshanense polysaccharide with anti-digestive system cancer effect
  • Dendrobium huoshanense polysaccharide with anti-digestive system cancer effect
  • Dendrobium huoshanense polysaccharide with anti-digestive system cancer effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] see figure 1 , a preparation of Dendrobium Huoshanense polysaccharide with anti-digestive system cancer effect is as follows:

[0046] (1) Dry the fresh stems of Dendrobium Huoshanense at 60°C, crush them, and sieve them through an 80-mesh sieve; add 95% ethanol according to the ratio of material to liquid: 1g:10 mL, stir at 25°C and 50rpm / min for 24 hours, and filter , poured off the ethanol, and the filter residue was naturally air-dried to obtain decolorized and degreased Dendrobium huoshanense stem powder;

[0047] (2) Mix the decolorized and degreased Dendrobium huoshanense stem powder with distilled water according to the material-to-liquid ratio of 1g:40mL, adjust the pH value to 5.0 with 0.1mol / L hydrochloric acid solution (HCl), add enzyme activity to 40000U / g, and the addition amount is Dendrobium Huoshanense stem powder with a mass fraction of 1.5% pectinase was stirred and enzymolyzed at 50°C and 50 rpm / min for 2 hours to obtain a pectinase pretreated produ...

Embodiment 2

[0058] The physical and chemical properties of Dendrobium Huoshanense polysaccharides with anti-cancer effects on digestive system are analyzed as follows:

[0059] (1) adopt the phenol-sulfuric acid method to measure the carbohydrate content of the polysaccharide of Dendrobium huoshanense obtained in embodiment 1

[0060] It has been determined that the carbohydrate content in the polysaccharide of Dendrobium huoshanense obtained in Example 1 is 97.20±2.56%, and the instrument used is a V1100 spectrophotometer.

[0061] (2) adopt UV scanning to measure the ultraviolet absorption of the polysaccharide of Dendrobium huoshanense obtained in embodiment 1

[0062] see figure 2 , after detection, no pigment, protein and nucleic acid absorption peaks in the UV scanning spectrum of the obtained Dendrobium huoshanense polysaccharide, the instrument used is a TU-1901 type ultraviolet-visible spectrophotometer, and the scanning range is 190~400nm.

[0063] (3) Measure the acetyl cont...

Embodiment 3

[0074] The chemical structure of a polysaccharide from Dendrobium Huoshanense with anti-digestive system cancer effect is identified as follows:

[0075] (1) The monosaccharide composition of the polysaccharide of Dendrobium huoshanense obtained in Example 1 was measured by the HPLC method of derivatization before the PMP column

[0076]Determination of correction factor: Accurately weigh an appropriate amount of glucosamine hydrochloride, add water to make a solution containing 12mg per 1mL, and use it as an internal standard solution; in addition, accurately weigh 10mg each of glucose and mannose reference substances, put them in 100mL volumetric flasks, and add them precisely Internal standard solution 1mL, add appropriate amount of water to dissolve and dilute to the mark, shake well, absorb 400μL, add 0.5mol / L PMP (1-phenyl-3-methyl-5-pyrazolinone) methanol solution and 0.3mol 400 μL each of NaOH solution per L, mix well, react in a water bath at 70°C for 100 min, add 500...

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Abstract

The invention relates to a Dendrobium huoshanense polysaccharide with an anti-digestive system cancer effect. The polysaccharide is an acetylated glucomannan having a novel structure and uniform components and obtained from the stems of Dendrobium huoshanense, the relative molecular mass is 2.59 * 10<5> Da, the repeating structural unit of the polysaccharide is formed by three sugar residues whichare -4)-beta-D-glucopyranose-(1-, -4)-beta-D-mannopyranose-(1- and -4)-3-oacetyl-beta-D-mannopyranose-(1-, and a molar ratio of glucose to mannose is 1:3. The Dendrobium huoshanense polysaccharide with the anti-digestive system cancer effect is prepared by combining a pectase and high temperature-resistant amylase hydrolysis process with an anion and cation exchange resin purification process, sothe pollution of pectin and starch is effectively avoided, and the removal of impurities such as proteins and salts is achieved; process conditions are mild, and the prepared polysaccharide has a high purity; and the Dendrobium huoshanense polysaccharide of the invention has small side effects, has resistance to digestive system cancers such as gastric cancer, colorectal cancer and liver cancer,and can be used for the development of new anti-digestive cancer drugs.

Description

technical field [0001] The invention relates to the field of polysaccharides of Chinese herbal medicines, in particular to a polysaccharide of Dendrobium huoshanense with anti-cancer effect of digestive system. Background technique [0002] Cancer is one of the global diseases that seriously threaten human health and life safety. Among them, the incidence and mortality of gastric cancer, liver cancer, colorectal cancer, and other digestive system cancers are increasing year by year, and the incidence rate of colorectal cancer is 10.2%. The incidence rate of gastric cancer is 5.7%, ranking third and fifth in the incidence of new cancers in the world, while the mortality rates of colorectal cancer, gastric cancer, and liver cancer are 9.2%, 8.2% and 8.2%, respectively, ranking the highest in global cancer deaths Ranked second to fourth. At present, cancer treatment including digestive system cancer is still dominated by surgery, supplemented by radiotherapy and chemotherapy, ...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/14C12P19/04A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003C08B37/006C12P19/04C12P19/14
Inventor 罗建平葛基春刘冰尚贞子张军王成卢润樟
Owner HEFEI UNIV OF TECH
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