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PCR rapid reaction chip

A rapid reaction, chip technology, applied in the fields of biology and analytical chemistry, can solve the problem that the chip cannot meet the demand, and achieve the effect of being beneficial to batch operation, reducing pollution and eliminating pollution.

Pending Publication Date: 2019-11-15
北京吉检医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the past, PCR testing equipment often started a new round of testing after the end of a round. In emergency applications, there are more and more demands for follow-up inspections, and the follow-up inspection function of PCR equipment is becoming more and more important. The current PCR rapid response chip cannot meet the demand

Method used

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Examples

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Embodiment Construction

[0031] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

[0032] The PCR rapid reaction chip 1 is composed of a channel plate 19 and a sealing plate 18, wherein the channel plate 19 is an intermediate layer. Such as image 3 with Figure 4 As shown, the channel sheet 19 is a film sheet, including at least one group of PCR reaction groups composed of liquid filling holes 11, vent holes 12 and PCR micro-reactio...

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Abstract

The invention relates to a PCR rapid reaction chip. The PCR rapid reaction chip comprises a channel piece and a sealing fin closely attached to the channel piece. The channel piece is a thin film piece and comprises at least one PCR reaction group composed of a liquid adding hole, an exhaust hole and a PCR micro reaction pool communicated with the liquid adding hole and the exhaust hole. The liquid adding hole and the exhaust hole are formed in the surface of the channel piece. The liquid adding hole is communicated with the PCR micro reaction pool through a liquid adding channel. The exhausthole is communicated with the PCR micro reaction pool through an exhaust channel. One face of the channel piece is provided with a hollowed-out structure which forms the PCR micro reaction pool and isopened towards the sealing fin. One face of the sealing fin is closely combined with the face, with the hollowed-out structure, of the channel piece and covers the hollowed-out structure to form a PCR micro reaction pool cavity. The PCR micro reaction pool cavity, the liquid adding channel and the exhaust channel are connected to form an integrated cavity. The sealing fin is made of a metal filmor a biological film. Due to the structural settings, the PCR rapid reaction chip can be matched with a heating device, so rapid heating and cooling are realized, and the PCR reaction time is shortened.

Description

technical field [0001] The invention relates to the fields of biology and analytical chemistry, in particular to a PCR quick reaction chip. Background technique [0002] PCR (polymerase chain reaction) is an important means of molecular biology research, the reaction time has always been the limiting factor of PCR reaction, a conventional PCR reaction often takes one or two hours, in clinical testing, especially for emergency, so The long detection time limits its application field. With the development and application of PCR fast reagents, the bottleneck of reaction time is mainly concentrated on the heating and cooling speed. Based on this, how to improve the heating and cooling speed of PCR reaction has become the key to its expanded application. In addition, in the past, PCR testing equipment often started a new round of testing after the end of a round. In emergency applications, there are more and more demands for follow-up inspections, and the follow-up inspection fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00
CPCB01L7/52B01L3/5027B01L2200/141B01L2300/1805
Inventor 王馥丽王宪华张伟刘旭王超徐辉柴旭阳
Owner 北京吉检医疗科技有限公司
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