Mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method

A technique for radiation-induced lung injury, analytical methods, applied in the field of radiation protection

Pending Publication Date: 2019-11-15
CHINA INST FOR RADIATION PROTECTION
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Problems solved by technology

For radiation-induced lung injury, the initial clinical treatment method is the use of adrenocorticosteroids combined with antibiotics. Although large doses of corticosteroids can temporarily relieve symptoms and inhibit the development of pulmonary fibrosis, many complications are prone to occur

Method used

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  • Mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method
  • Mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method
  • Mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method

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Embodiment 1

[0025] 1. Experimental animals

[0026] Ten 6-8-week-old SPF male SD rats, weighing (205±15) g, were provided by Beijing Weitong Lihua Experimental Animal Company [SCXK (Beijing) 2016-0006]. The animals were kept in the animal laboratory of the GLP Center of the Chinese Institute of Radiation Protection [SYXK (Jin) 2013-0002].

[0027] 2. Establishment of a model of radiation-induced lung injury in rats protected by mild hypothermia

[0028] Ten SD rats were randomly divided into two groups: model group and mild hypothermia treatment group, with 5 rats in each group. After being anesthetized by intraperitoneal injection of 10% chloral hydrate, the patient was placed supine under a medical linear accelerator, and 20Gy vertical single irradiation was performed with a 12MeV electron beam. The source-to-skin distance was 100cm, the irradiation depth was 3cm, and the dose rate was 300cGy / min. The model group was positioned and irradiated after anesthesia, and the rats in the mild...

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Abstract

The invention belongs to the technical field of radiation protection, and relates to a mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method. Themethod includes the following steps of firstly, conducting mild-hypothermia intervention treatment on an established rat radiation-induced pulmonary injury model; secondly, screening out a differential expression gene of the mild-hypothermia treatment radiation-induced pulmonary injury after treatment, and conducting enrichment analysis on the functions of the gene; thirdly, selecting a differential expression remarkable gene after analysis for result verification. By means of the mild-hypothermia protection rat radiation-induced pulmonary injury gene screening and path analyzing method, the mild-hypothermia protection rat radiation-induced pulmonary injury related gene can be screened out, the function and path enrichment analysis is conducted on the differential expression gene, and theexplanation on the gene level is provided for the mild-hypothermia protection rat radiation-induced pulmonary injury.

Description

technical field [0001] The invention belongs to the technical field of radiation protection, and relates to a gene screening and pathway analysis method for mild hypothermia protection against radiation-induced lung injury in rats. Background technique [0002] The national cancer statistics report for many years has pointed out that: lung cancer continues to be the first in the incidence and mortality of cancer in my country, and breast cancer has the highest incidence rate among female cancers, and the most common part of cancer is still located in the chest. [0003] In the case of continued high incidence of cancer, multimodal diagnosis and treatment methods for patients with primary chest tumors and metastatic tumors have greatly improved the survival rate and prolonged the survival time of patients, especially radiotherapy, which has become one of the main treatment methods for cancer patients . However, while radiotherapy kills tumor cells, it will also cause damage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6809
CPCC12Q1/6809C12Q2539/103
Inventor 王远飞李曙芳王新钢许文黎安全李建国秦秀军王永丽刘欢郭剑平黄立群岳娟尹晶晶袁慧刘梦雅杨彪张亚娟
Owner CHINA INST FOR RADIATION PROTECTION
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