DNA probe structure and manufacturing method thereof

A technology of DNA probes and production methods, applied in the direction of material excitation analysis, fluorescence/phosphorescence, instruments, etc., to achieve the effect of light weight, good effect and small structure

Active Publication Date: 2019-11-15
SHENZHEN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using molecular beacons as probe DNA, the process of preparing optical fiber SERS gene probes is relatively simple, and there is currently no such DNA probe and production method

Method used

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  • DNA probe structure and manufacturing method thereof
  • DNA probe structure and manufacturing method thereof
  • DNA probe structure and manufacturing method thereof

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Embodiment 1

[0031] The optical fiber 1 is one of ordinary refractive index gradient type, refractive index graded single-mode fiber, multimode fiber, and silica fiber with a core size of 10 microns to 600 microns, preferably a silica fiber with a core size of 200 μm. For the optical fiber, the metal nanoparticle substrate 2 includes but not limited to spherical, triangular, plate-shaped, and cubic metal particles. In this embodiment, the metal nanoparticle material is silver. The probe sensitive layer 3 includes, A single-stranded DNA molecule 301 with complementary ends, one end is modified with a sulfhydryl (-S) or sulfhydryl (-SH) functional group 302, and one end is modified with a fluorescent molecule 303, the fluorescent molecule 303 can be FAM, Cy5, ROX, etc., This kind of DNA molecule coils into a hairpin structure in a free state.

[0032] The above-mentioned tapered optical fiber Raman DNA probe can be made by the following steps:

[0033] Such as image 3 As shown in , the op...

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Abstract

The invention, which is suitable for the field of optical fiber sensing, provides a tapered optical fiber Raman DNA probe structure and a manufacturing method thereof. The probe structure comprises apyramidal optical fiber substrate 1 with a roughened surface, a metal nanoparticle reinforced substrate 2 and a DNA probe sensitive layer 3 with a hairpin structure. In addition, the invention also provides a manufacturing method of the tapered optical fiber Raman DNA probe structure. The method includes: making a pyramidal fiber 1 with a roughened surface by using a femtosecond laser micromachining technology; on the basis of an electrostatic adsorption or light-induced growth method, modifying the surface of the tapered fiber with metal nanoparticles 2 and growing the metal nanoparticles 2 on the surface of the tapered fiber; and then assembling a DNA probe sensitive layer 3 with a hairpin structure to realize a highly sensitive optical fiber DNA probe. According to the invention, the tapered optical fiber Raman DNA probe structure has advantages of simple structure and high sensitivity; and high-sensitivity specific detection of DNA target fragments is realized.

Description

technical field [0001] The invention belongs to the field of optical fiber sensing, in particular to a tapered optical fiber Raman DNA probe and a manufacturing method. Background technique [0002] As the carrier of genetic information, genes can control the traits of individual organisms by guiding protein synthesis, and pass genetic information to offspring through replication. Therefore, gene analysis and detection have important application value in the fields of medical diagnosis and eugenics. For diseases that seriously affect human health, more than half of the symptoms are related to genetic genes; therefore, it is of great significance to realize the clinical detection of disease-causing genes. So far, there are many methods for genetic detection, such as high-throughput sequencing and melting curve analysis , denaturing liquid chromatography analysis, etc., but these methods are cumbersome or difficult to achieve clinical detection. In contrast, fiber optic DNA pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/65
CPCG01N21/645G01N21/6428G01N21/658
Inventor 耿优福许译文王丽娜易多洪学明李学金
Owner SHENZHEN UNIV
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