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Methods and systems using ionophores to control contamination in fermentation of gaseous substrates

A gaseous substrate, ionophore technology, applied in the biological field to minimize the growth of fermented species and/or produce liquid products, and can solve the problems of low efficiency and high cost

Pending Publication Date: 2019-11-15
SYNATA BIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process is costly and inefficient

Method used

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  • Methods and systems using ionophores to control contamination in fermentation of gaseous substrates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0218] Contamination control with 20μM slug dose (Slug Dose) under steady state operation

[0219] An ionophore, such as a sodium-specific ionophore (eg, monensin), is added to a 2 liter bioreactor containing homoacetogenic bacteria such as Clostridium ethanologen. Experiment A is a control. To the bioreactor in experiment B, a competing species of only acetate producers, such as Acetobacter wooderii, was added. Experiment B proved that ionophores can be used to inhibit the targeted population of microorganisms in the bioreactor. Experiment C utilized a homoacetogen reactor contaminated with a competing species supplied via an unknown environmental sample. Experiment C proves that the ionophore is more selective than a variety of contaminants for process homoacetogens.

[0220] Experiment A

[0221] Once the steady state operation of the bioreactor is reached, the ionophore is added as a slug dose (20 μM total). The bioreactor contains fermenting species, such as Clostridium eth...

Embodiment 2

[0227] Use continuous dose for pollution control under steady state operation

[0228] An ionophore, such as a sodium-specific ionophore (eg, monensin), is added to a 2 liter bioreactor containing homoacetogenic bacteria such as Clostridium ethanologen. Experiment D is a control. To the bioreactor in Experiment E was added a competing species of only acetate producers, such as Acetobacter wooderii. Experiment E proves that ionophores can be used to inhibit the targeted population of microorganisms in the bioreactor. Experiment F utilized a homoacetogen reactor contaminated with a competing species supplied via an unknown environmental sample. Experiment F proves that the ionophore is more selective than a variety of pollutants for process homoacetogens.

[0229] Experiment D

[0230] Once the steady state operation of the bioreactor is reached, the ionophore is added as a continuous dose, and its final concentration in the bioreactor is 20 μM. The bioreactor contains fermenting ...

Embodiment 3

[0236] Use of slug dose for pollution control at start-up of the bioreactor

[0237] An ionophore, such as a sodium-specific ionophore (eg, monensin), is added to a 2 liter bioreactor containing homoacetogenic bacteria such as Clostridium ethanologen. Experiment G is a control. To the bioreactor in Experiment H was added a competing species of only acetate producers, such as Acetobacter wooderii. Experiment H proved that ionophores can be used to inhibit the targeted population of microorganisms in the bioreactor. Experiment I utilized a homoacetogen reactor contaminated with a competing species supplied via an unknown environmental sample. Experiment I proved that the ionophore is more selective than a variety of contaminants for process homoacetogens.

[0238] Experiment G

[0239] The ionophore was added to one of the tanks as a slug dose of 5 μM at the start of the bioreactor. The bioreactor contains fermenting species, such as Clostridium ethanologen. The results show that...

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Abstract

Provided is a method of anaerobically fermenting a gaseous substrate to form a liquid product, the method comprising: (a) introducing the gaseous substrate into a bio-reactor, the gaseous substrate comprising at least one of the following constituents: carbon monoxide, carbon dioxide, and hydrogen, (b) the bio-reactor comprising a fermentation broth therein, the fermentation broth containing at least two types of microorganisms, one type comprising at least one fermenting species, and the other type comprising at least one competing species; (c) introducing at least one type of ionophore intothe reactor, the ionophore having selectivity for preferentially inhibiting the at least one competing species from growing and / or producing an undesired product; and (d) allowing the gaseous substrate to ferment by exposure to the at least one fermenting species, to produce the liquid product and a system for doing the same.

Description

[0001] Cross references to related applications [0002] This patent application claims the priority rights of U.S. Provisional Patent Application No. 62 / 438,211 entitled "Methods And Systems Using Ionophores To Control Contamination In Fermentation Of Gaseous Substrates" filed on December 22, 2016, which is incorporated by reference in its entirety This article. Background technique [0003] Ethanol is the main source of biofuels worldwide, and therefore, new methods of bioethanol production are highly sought after. Generally, biofuels, and more specifically bioethanol, are produced by the fermentation of sugars derived from, for example, corn, wheat, and sugar cane. This method is very desirable because the use or conversion of biomass is a renewable and potentially sustainable source of energy production. However, due to competition with food production, available land and water use, and other factors, carbohydrates that are easily fermentable are limited. [0004] An efficient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/14C12P7/04C12P7/16C12P7/40C12P7/54
CPCC12P7/04C12P7/065C12P7/14C12P7/16C12P7/40C12P7/54Y02E50/10C12M21/12C12M43/02
Inventor 詹妮·B·泰瑞尔
Owner SYNATA BIO INC