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Method for emerging of potential fingerprints through enzymatic reaction system

An enzyme-catalyzed reaction and system technology, applied in applications, medical science, sensors, etc., can solve the problems of complex preparation process, complicated operation process, and long time consumption, and achieve the effect of uncomplicated composition, simple display and operation, and low cost

Active Publication Date: 2019-11-19
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the Chinese patent (CN201810189499.3), the inventor prepared a magnetic fluorescent powder for latent fingerprint display to improve sensitivity, but the preparation process is complicated
Chinese patent (CN201710888373.0) prepared a special material: black body radiation luminescent material, which can reduce the background fluorescence to obtain a clearer fingerprint image, but the preparation process of the material is complicated
Chinese patent (CN201710045379.1) uses enzymes to visualize latent fingerprints, but it needs to transfer latent fingerprint samples twice, and then use relevant reagents to visualize after labeling with antibodies. The whole operation process is very complicated and consumes duration
[0005] At present, there is no report on the development of latent fingerprints by enzymatically catalyzing the reaction of trace substances in latent fingerprints in a short period of time under normal temperature and pressure.

Method used

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  • Method for emerging of potential fingerprints through enzymatic reaction system
  • Method for emerging of potential fingerprints through enzymatic reaction system
  • Method for emerging of potential fingerprints through enzymatic reaction system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of method that enzymatic reaction system is used for latent fingerprint revealing, its steps are as follows:

[0034] 1. Sample preparation of natural fingerprints: Please clean the hands of the tester who provided the fingerprints, dry them by themselves, then put on plastic gloves, cover the sweat for 10 minutes, press the finger on the writing paper without fluorescent components, and use it after 4 hours (Simulate the actual situation of criminal investigation and case handling).

[0035] 2. Preparation of chromogenic solution:

[0036] 1) Tris-HCl-hydrazine hydrate buffer (the concentration of hydrazine hydrate is 1.5mol / L): add 5mL0.1mol / L HCl solution to 25mL0.2mol / L Tris solution, add water to 100mL, and the pH is 9.0, 0.05mol / L Tris-HCl buffer solution, then add 9.38mL85% (mass percentage, the same below) hydrazine hydrate solution to the buffer solution to obtain a Tris-HCl-hydrazine hydrate buffer solution with a hydrazine hydrate concentration of 1....

Embodiment 2

[0043] A method for developing latent fingerprints using an enzymatic reaction system adding glycerol, the steps of which are the same as those in Example 1, the only difference being the preparation of the buffer.

[0044] This example uses Tris-HCl-glycerol-hydrazine hydrate buffer solution (the concentration of hydrazine hydrate is 1.5 mol / L): add 5 mL of 0.1 mol / L HCl solution to 25 mL of 0.2 mol / L Tris solution, add 20 mL of glycerin, and then add water to 100 mL , to get pH 9.0, 0.05mol / LT Tris-HCl-glycerol buffer solution, and then add 9.38mL85% hydrazine hydrate solution to the buffer solution to get Tris-HCl-glycerol-hydration with hydrazine hydrate concentration of 1.5mol / L Hydrazine buffer.

[0045] From figure 2It can be seen that clear fingerprint lines can be shown on the writing paper.

Embodiment 3

[0047] A method of adding PEG400 enzymatic reaction system for displaying latent fingerprints, the steps of which are the same as in Example 1, the only difference is that the preparation of buffer is different.

[0048] This example uses Tris-HCl-PEG-hydrazine hydrate buffer solution (the concentration of hydrazine hydrate is 1.5mol / L): add 5mL0.1mol / L HCl solution to 25mL0.2mol / L Tris solution, add 15mLPEG400, and then add water to 100mL, Promptly get pH9.0, 0.05mol / LTris-HCl-PEG buffer solution, then add 9.38mL85% hydrazine hydrate solution to the buffer solution to get Tris-HCl-PEG-hydrazine hydrate with hydrazine hydrate concentration of 1.5mol / L buffer.

[0049] From image 3 It can be seen that clear fingerprint lines can be shown on the writing paper.

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Abstract

The invention relates to the technical field of trace detection, in particular to a method for emerging of potential fingerprints through an enzymatic reaction system. The method is a method for emerging of the potential fingerprints through fluorescent products generated by an enzymatic reaction. According to the method, firstly, an enzymatic reaction system developing solution is prepared, thena fingerprint object with possible fingerprints and without fluorescent substances is sprayed or coated with the developing solution, enzymes and other substances in the system will react with lacticacid in the fingerprints, the fluorescent products are generated, accordingly, the fingerprints emerge, and under irradiation of ultraviolet, direct shooting can be conducted. The method has the advantages that sensitivity and rapidness are realized, the compositions of the developing reagent are simple, an operator will not be poisoned, hidden observation can be further realized, and the method is expected to be applied in the aspects of criminal investigation and others.

Description

technical field [0001] The invention relates to the technical field of trace detection, in particular to a method for an enzymatic reaction system for latent fingerprint visualization. Background technique [0002] Fingerprints are different from person to person, remain unchanged for life and leave traces on objects touched. Scientifically and correctly discovering, extracting, displaying, and identifying fingerprints has important practical functions for carrying out investigation work and punishing crimes. Extracting and identifying fingerprints left on the surface of objects is an important basis for solving cases. [0003] At present, the methods for identifying latent fingerprints can be divided into three categories: physical visualization method, physicochemical visualization method and chemical visualization method. The development and improvement of current technology mainly focus on the use of novel characterization methods to increase the sensitivity of inspectio...

Claims

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Application Information

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IPC IPC(8): A61B5/1172
CPCA61B5/1172
Inventor 林爱华赵燕熹王利华蒙永江张鹏宋发军耿红王红莹孟艳艳
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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