Three-dimensional volume imaging method and device based on compressed sensing and random multi-focus scanning
A technology of compressed sensing and imaging devices, which is applied in measurement devices, optical devices, image data processing, etc., can solve problems such as signal decomposition, and achieve the effect of high temporal resolution and high spatial resolution
Active Publication Date: 2020-11-13
TSINGHUA UNIV
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However, multi-focus scanning makes the information on multiple points aliased together, and the biggest problem is how to decompose the aliased signals
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[0054] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0055] The 3D volume imaging method and device based on compressed sensing and random multi-focus scanning according to the embodiments of the present invention will be described below with reference to the accompanying drawings.
[0056] First, a three-dimensional volume imaging method based on compressed sensing and random multi-focus scanning proposed according to an embodiment of the present invention will be described with reference to the accompanying drawings.
[0057] figure 1 It is a flowchart of a three-dimensional vol...
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The invention discloses a three-dimensional body imaging method and device based on compressed sensing and random multi-focus scanning. The method includes the following steps: in a pre-built imagingsystem, generating an ultra-short laser pulse through a femtosecond pulsed laser, and conjugating a spatial light modulator to a back focal plane of an object lens by utilizing multiple 4f imaging systems; according to a minimum mean square error loss function and an ADAM non-convex optimization algorithm, generating multiple phase patterns and displaying the patterns in sequence on the spatial light modulator, generating multiple focus positions corresponding to each of the phase patterns on a sample by the back focal plane of the object lens, performing multi-time scanning on the multiple focus positions corresponding to each of the phase patterns to generate a sample information image sequence; and constructing a detection matrix, and performing reconstruction pixel by pixel on the sample information image sequence according to the detection matrix and a compressed sensing sparse reconstruction algorithm to generate three-dimensional information of the sample. The method has the advantages of being wide in field of view, being high in temporal resolution, being high in spatial resolution, and being suitable for video rate three-dimensional body imaging.
Description
technical field [0001] The invention relates to the technical fields of optical microscopic imaging and computational imaging, in particular to a three-dimensional volume imaging method and device based on compressed sensing and random multi-focus scanning. Background technique [0002] Optical microscopy imaging technology is a non-invasive observation technology, which is widely used in the structural and functional imaging of biological tissues, and has been widely used in the field of neuroscience. Combined with fluorescent labeling technology, fluorescence microscopy can label specific structures and substances, thereby excluding interference from other structures and achieving specific imaging. Traditional single-photon microscopy has disadvantages such as strong background fluorescence, lack of tomographic ability, and severe photobleaching; light sheet microscopy reduces photobleaching by placing excitation and detection in two mutually perpendicular directions, and ...
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IPC IPC(8): G01N21/84G01N21/01G01B11/00G06T17/00
CPCG01B11/00G01N21/01G01N21/84G01N2021/0112G06T17/00
Inventor 戴琼海李欣阳张元龙孔令杰王好谦
Owner TSINGHUA UNIV