Detection method and application of antimicrobial in a kind of sample

Abstract

The invention discloses a detection method of an antibacterial agent in a sample and an application thereof. The method comprises the following steps: respectively extracting a sample and a blank control by adopting an acidic aqueous solution, water and an alkaline aqueous solution to obtain a to-be-detected sample extracting solution and a control extracting solution, respectively detecting the minimum inhibitory concentrations (MIC) of the to-be-detected sample extracting solution and the control extracting solution, and performing comparison to obtain whether the antibacterial agent existsor not. Wherein the sample is a plant extract or an additive containing the plant extract; the blank control is a supersaturated solution thereof having the same plant extract as the sample. The method aims at the difference of solubility of the sample and the antibacterial agent in acid and alkali aqueous solutions. Whether the antibacterial agent is added into the sample or not can be judged bymeasuring the minimum bacteriostatic concentrations of the extracting solutions with different pH values and comparing the minimum bacteriostatic concentrations with the MIC of the contrast extractingsolution, and the method is simple to operate, convenient, rapid, low in cost and capable of rapidly checking whether the antibacterial agent is added into plant extract additives or health-care medicines or not.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for detecting antibacterial drugs in a sample. Background technique [0002] After the photosynthesis of plants, the cells secrete aromatic molecules, which gather into sachets and spread them on petals, leaves or trunks. After the sachets are refined and extracted, they become "plant essential oils", also known as plant extracts. Plant extracts are widely used in daily life because of their wide variety and wide range of functions. The American Food Flavor and Extraction Manufacturers Association (FEMA) has determined that natural plant essential oils are safe and pollution-free substances. [0003] Plant essential oils have biological functions such as promoting animal feed intake, improving production performance, anti-oxidation, and enhancing body immunity. For example, Marcincak et al. found that the average body weight of broilers increased by 4.1% compare...

AI Technical Summary

Problems solved by technology

For plant extract products, the existing detection methods are suitable for accurately knowing which antibacterial drug is contained in the sample. Such detection not only requires the use of special, expensive, and large-scale equipment, but the detection cost is high and time-consuming. It is impossible to conduct primary screening of multiple antibacterial drugs at the same time, and it is also difficult to realize rapid and simple detection of large quantities of samples, so that it is impossible to quickly judge whether antibacterial drugs are illegally added to plant extract products. In actual promotion and application, professional Before accurately detecting how much and which kind of antibacterial drugs are contained in the sample, it is more necessary to quickly and easily understand whether antibacterial drugs are added to the sample (adding an effective amount in violation of regulations). A detection method for antibacterial drugs and the content of antibacterial drugs. Due to the detection requirements and the need for special technology and equipment, the existing detection methods cannot be popularized and implemented.

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