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A spatula-based electrochemical biosensor

A biosensor and electrochemical technology, applied in the field of biosensing, can solve the problems of high cost, incompetence of detection instruments, large and complex instruments, etc., and achieve the effect of high accuracy and stable current

Active Publication Date: 2021-05-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional biosensors often have inherent defects such as large and complex instruments, high cost, cumbersome operation and the need for professionals, and long information feedback time.
In the emerging fields of home medicine and POCT (point of care testing), patients and nursing staff need an instrument that is cheap, easy to use, and has rapid and accurate data reading to detect the concentration of various target analytes in saliva. Faced with these requirements, traditional testing instruments are often incompetent
At present, the traditional biological detection method still uses large-scale optical detection instruments, and such detection methods can only be completed by hospitals, and it is difficult to enter more grass-roots health institutions and ordinary families. Electrochemical biosensors are easy to manufacture, fast in feedback, portable and cheap Opens up new ideas for solving the popularization of biological testing

Method used

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  • A spatula-based electrochemical biosensor
  • A spatula-based electrochemical biosensor
  • A spatula-based electrochemical biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] image 3 H measured for the electrochemical biosensor of the present invention in embodiment one 2 o 2 Detection representation diagram. In this embodiment, an electrochemical workstation is used as a power source to detect the spatula sensor H 2 o 2 concentration test. image 3 H is shown using an electrochemical workstation and based on the electrochemical biosensor of the present invention 2 o 2 detected features. Use an electrochemical workstation to measure at 0.6V, place 200 μl of 50 mM buffer solution on the wearable sensor, and then add 10 μl of various concentrations of H 2 o 2 solution; let the mixture stand until the current stabilizes, then continue to add the next concentration of H 2 o 2 . image 3 Among them, C1~C8 are the concentration changes of the detected substances in the solution after the dropwise addition (the same below): C1:0.1mM, C2:0.2mM, C3:0.2mM, C4:0.5mM, C5:1.0mM, C6: 1.0mM, C7: 1.0mM, C8: 1.0mM. image 3 a shows the H 2 o ...

Embodiment 2

[0059] Figure 4 It is the glucose detection characterization graph measured by the electrochemical biosensor of the present invention in Example 2. In this embodiment, an electrochemical workstation was used as a power source to conduct a test for detecting glucose concentration using the electrochemical biosensor of the present invention. Figure 4 Shown is the time-current curve of using the electrochemical workstation as an instrument to detect lactic acid with the electrochemical biosensor of the present invention. Set the voltage of the electrochemical workstation to 0.6 V, then add 200 μl of 50 mM buffer solution, and add 10 μl of each concentration of glucose solution on top of the working electrode. After waiting for the current to stabilize, continue to drop the next concentration of lactic acid. Figure 4Among them, C1~C10 are the concentration changes of the detected substances in the solution after the dropwise addition (the same below): C1:0.1mM, C2:0.2mM, C3:0...

Embodiment 3

[0061] Figure 5 It is the glucose detection characterization graph measured by the electrochemical biosensor of the present invention in Example 3. In this embodiment, the glucose in the saliva of a real person is detected, and 180 μl of a healthy male saliva sample is taken as a buffer solution, and 20 μl of a glucose solution with a concentration of 1 mM is added dropwise thereto to increase the total glucose concentration by 0.1 mM, that is, 100 μM. The ability of the electrochemical biosensor of the present invention to distinguish saliva from healthy and diabetic patients was tested. The average current response of the electrochemical biosensor of the present invention to a glucose concentration change of 0.1 mM is 0.20 μA, the standard deviation is 0.011 μA, and the concentration detection value corresponding to the standard working curve is 93.1±5.1 μM.

[0062] The electrochemical biosensor based on spatula provided by the present invention can detect the glucose con...

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Abstract

The invention discloses an electrochemical biosensor based on a spatula and a sensing method thereof. The electrochemical biosensor is based on a disposable wooden spatula and is equipped with a power supply (1), a computing circuit (2) and The display screen (6) has a three-electrode electrochemical system on the other side, including a reference electrode RE (3), a counter electrode CE (4) and a working electrode WE (5). The electrochemical sensing part and the electrical signal processing display part are common Constitutes a complete portable biosensing device; the electrochemical biosensor is convenient and fast to detect, can be carried around, and can obtain the concentration of saliva glucose at any time. Cross-infection does not rely on user interpretation, accurate readings, and higher detection accuracy.

Description

technical field [0001] The invention belongs to the technical field of biosensing, and in particular relates to a spatula-based electrochemical biosensor for saliva analytes and a sensing method thereof. Background technique [0002] Current biosensors have been successfully applied in many fields, including medical treatment, environmental monitoring, and national defense science and industry. However, traditional biosensors often have inherent defects such as large and complex instruments, high cost, cumbersome operation and the need for professionals, and long information feedback time. In the emerging fields of home medicine and POCT (point of care testing), patients and nursing staff need an instrument that is cheap, easy to use, and has rapid and accurate data reading to detect the concentration of various target analytes in saliva. Faced with these requirements, traditional testing instruments are often incompetent. At present, the traditional biological detection m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/3271G01N27/3272
Inventor 崔悦石蔚骅罗小进刘逸群
Owner PEKING UNIV