Preparation method of calcium carbonate coated DNA-fluorescent dye composite nano gel dual anti-counterfeiting coating

A fluorescent dye and nanogel technology is applied in the field of preparation of calcium carbonate-coated DNA-fluorescent dye composite nanogel double anti-counterfeiting coatings, which can solve the problems of complicated and time-consuming operations, samples that cannot be reused, lack of clear anti-counterfeiting methods, and the like. High biocompatibility effect

Active Publication Date: 2019-11-26
湖南省南盾防伪技术实业有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above-mentioned patents have achieved effective DNA anti-counterfeiting, DNA anti-counterfeiting is dark anti-counterfeiting, and it is necessary to destroy the sample and extract and amplify the DNA during the measurement. Professional equipment such as electrophoresis or even a sequencer is required. The operation is complicated and time-consuming, and the sample cannot be tested after the measurement Re-use, lack of a simple anti-counterfeiting method that does not destroy the sample to cooperate with it

Method used

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  • Preparation method of calcium carbonate coated DNA-fluorescent dye composite nano gel dual anti-counterfeiting coating
  • Preparation method of calcium carbonate coated DNA-fluorescent dye composite nano gel dual anti-counterfeiting coating
  • Preparation method of calcium carbonate coated DNA-fluorescent dye composite nano gel dual anti-counterfeiting coating

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Embodiment 1

[0029] according to figure 1 The shown route prepares calcium carbonate-wrapped DNA-fluorescent dye composite nanogel light and dark double anti-counterfeiting coatings, and the specific steps are as follows:

[0030] Step 1: Collect the DNA sequence from the plasmid DNA, use the Primer Pemier 5.0 software to design primers, and intercept and amplify a DNA fragment with a length of 720 bp by PCR. The DNA amplification conditions are as follows: 50 μL system, including 25 μL Taq DNA polymerase mixture, 1 μL 1 mg / mL template DNA, 20 μmol / L upstream and downstream primers, 1 μL each and 22 μL deionized water; the amplification procedure refers to Figure 5 A: Pre-denaturation at 95°C for 10 minutes; denaturation at 95°C for 60 seconds, annealing at 60°C for 60 seconds, extension at 72°C for 60 seconds, repeat 25 cycles; extension at 72°C for 5 minutes to complete the extension of the amplified DNA sequence and terminate the reaction.

[0031]Step 2: Configure gelatin solution at...

Embodiment 2

[0039] according to figure 1 The route shown prepares calcium carbonate-wrapped DNA-fluorescent dye composite nanogel double anti-counterfeiting coating, and the specific steps are as follows:

[0040] Step 1: Collect DNA sequence from λ-DNA, use Primer Pemier 5.0 software to design primers and intercept and amplify a DNA fragment with a length of 200 bp by PCR. DNA amplification conditions are as follows: 50 μL system, including 25 μL TaqDNA polymerase mixture, 1 μL 1mg / mL template DNA, 20 μmol / L upstream and downstream primers 1 μL each and 22 μL deionized water; amplification program: 95 ° C pre-denaturation for 10 minutes Denaturation at 95°C for 60 seconds, annealing at 60°C for 60 seconds, extension at 72°C for 60 seconds, repeated for 25 cycles; extension at 72°C for 5 minutes to complete the extension of the amplified DNA sequence and terminate the reaction.

[0041] Step 2: Prepare a gelatin solution at a concentration of 10% (w / v), and dissolve it completely at 40°C...

Embodiment 3

[0049] according to figure 1 The shown route prepares calcium carbonate-wrapped DNA-fluorescent dye composite nanogel light and dark double anti-counterfeiting coatings, and the specific steps are as follows:

[0050] Step 1: Collect the DNA sequence from the plasmid DNA, use the Primer Pemier 5.0 software to design primers, and intercept and amplify a DNA fragment with a length of 350 bp by PCR. DNA amplification conditions are as follows: 50 μL system, including 25 μL Taq DNA polymerase mixture, 1 μL 1 mg / mL template DNA, 20 μmol / L upstream and downstream primers 1 μL and 22 μL deionized water; amplification program: 95 ° C pre-denaturation for 10 minutes Denaturation at 95°C for 60 seconds, annealing at 60°C for 60 seconds, extension at 72°C for 60 seconds, repeated for 25 cycles; extension at 72°C for 5 minutes to complete the extension of the amplified DNA sequence and terminate the reaction.

[0051] Step 2: Prepare a gelatin solution at a concentration of 5% (w / v), and...

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Abstract

The invention relates to a preparation method of a calcium carbonate coated DNA-fluorescent dye composite nano gel dual anti-counterfeiting coating. The preparation method comprises the following steps: firstly, mixing DNA and a fluorescent dye with a gelatin solution, adding a cross-linking agent for cross-linking curing to coat the DNA and the fluorescent dye in a gelatin nano gel grid, and thencoating the nano gel with calcium carbonate to obtain the calcium carbonate coated DNA-fluorescent dye composite nano gel, so that the DNA and the fluorescent dye are isolated from the outside, and the light-dark dual anti-counterfeiting coating with long storage period, stable structure and easy detection is prepared. Light anti-counterfeiting is realized by the fluorescent dye, and dark anti-counterfeiting is realized by the DNA. Due to the unknown property and expandability of the DNA, the DNA can be detected through polymerase-chain reaction amplification and electrophoresis analysis onlyby knowing the DNA sequence and designing a specific primer, and thus dark anti-counterfeiting is realized. According to the invention, the purposes of difficult matching and cooperative anti-counterfeiting are achieved, and the anti-counterfeiting effect and the counterfeiting difficulty are greatly increased.

Description

technical field [0001] The invention relates to a method for preparing an anti-counterfeiting paint, in particular to a method for preparing a calcium carbonate-wrapped DNA-fluorescent dye composite nanogel double anti-counterfeiting paint. Background technique [0002] In recent years, the manufacture and sale of counterfeit goods has become increasingly rampant, seriously damaging normal business practices and consumer interests, and seriously restricting the healthy development of the national economy and the country's image. Therefore, anti-counterfeiting technology emerges at the historic moment thereupon. The so-called anti-counterfeiting means that one item has one code, and specific technical measures, materials, and technical equipment are used to identify authenticity and prevent counterfeit and counterfeit products. Traditional anti-counterfeiting technologies include: laser anti-counterfeiting, digital anti-counterfeiting, texture anti-counterfeiting, fluorescen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21H21/44D21H19/38D21H19/44
CPCD21H21/44D21H19/38D21H19/385D21H19/44
Inventor 林国平
Owner 湖南省南盾防伪技术实业有限公司
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