Chimeric antigen receptor and chimeric antigen receptor T cell targeting prostate specific membrane antigen (PSMA), preparation method of chimeric antigen receptor T cells and applications of chimeric antigen receptor and chimeric antigen receptor T cells

A chimeric antigen receptor and targeting technology, applied in the field of medical biology, can solve the problems such as the lack of relevant research and limitations of various PSMA-positive solid tumors, and achieve the effects of high expression, efficient transfer, and promotion of expansion.

Inactive Publication Date: 2019-12-03
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current application of CART technology is still limited to hematological tumors, and there is no relevant research on the application of various PSMA-positive solid tumors.

Method used

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  • Chimeric antigen receptor and chimeric antigen receptor T cell targeting prostate specific membrane antigen (PSMA), preparation method of chimeric antigen receptor T cells and applications of chimeric antigen receptor and chimeric antigen receptor T cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A method for preparing chimeric antigen receptor T cells targeting PSMA, comprising the following steps:

[0079] (1) Prepare the gene sequence of the chimeric antigen receptor CAR-PSMA targeting PSMA

[0080] The genes encoding the signal peptide, the single-chain antibody targeting PSMA, the hinge region of CD8α, the transmembrane region of CD8, the signal region of CD27, and the signal region of CD3ζ were respectively prepared, and the encoding gene of the signal peptide is shown in SEQ ID NO: 14. The coding gene of the single-chain antibody targeting PSMA is shown in SEQ ID NO: 5, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO 9, the coding gene of the CD27 signal region is shown in SEQ ID NO: 10, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 12.

[0081] The genes encoding the signal peptide, PSMA-targeting single-chain antibody, CD8α hinge region, ...

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Abstract

The present invention provides a chimeric antigen receptor targeting prostate specific membrane antigen (PSMA) and a chimeric antigen receptor T cell targeting the PSMA. The chimeric antigen receptortargeting the PSMA can specifically target the PSMA, also uses a CD27 signal region as a co-stimulatory signal region, promotes expansion of the T cells in patients, can efficiently and specifically kill PSMA-positive tumor cells, better maintains cell viability and lethality, and also does not cause damages to normal cells . The present invention also provides a preparation method and an application of the chimeric antigen receptor T cell targeting the PSMA.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a PSMA-targeted chimeric antigen receptor and chimeric antigen receptor T cells as well as a preparation method and application thereof. Background technique [0002] Prostate cancer is the most common malignant tumor of the male reproductive system. In the United States, the incidence of prostate cancer ranks first among all malignant tumors, and the mortality rate ranks second, second only to lung cancer. Although the incidence of prostate cancer in my country is far lower than that in western countries, it has shown a significant increase in recent years. Prostate specific membrane antigen (PSMA) is a newly discovered prostate cancer-associated antigen, which has strong prostate tissue specificity and has become an important target protein in the diagnosis and treatment of prostate cancer. There are not many PSMAs on normal prostate cells, but when the cells become cancerous, g...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N5/10C12N15/62C12N15/867A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/7051C07K16/3069C07K2317/622C07K2319/02C07K2319/03C07K2319/33C12N15/86C12N2510/02C12N2740/15043
Inventor 万晓春刘绿艳唐超李欣
Owner SHENZHEN BINDEBIOTECH CO LTD
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