Polyacrylamide gel for separating small-molecular-weight proteins and preparation method of polyacrylamide gel
A polyacrylamide gel and protein separation technology, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problem of high cost of buffer reagents, and achieve the effects of reduced preparation costs, good separation, and reduced use costs.
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Embodiment 1
[0032] A polyacrylamide gel for the separation of small molecular weight proteins, the gel includes acrylamide, N,N-methylenebisacrylamide, ultrapure water, gel buffer, glycerin, ammonium persulfate solution and tetramethyl ethylenediamine, the gel buffer includes boric acid and Tris-HCl solution, the pH of the gel buffer is 7.0, the concentration of Tris is 50-200mM, and the concentration of boric acid is 50-200mM.
[0033] The present invention also provides a method for preparing polyacrylamide gel used for the separation of small molecular weight proteins, comprising the following steps:
[0034] Step 1: prepare 30% acrylamide stock solution, wherein the mass ratio of acrylamide to N,N-methylene bisacrylamide is 29:1, the specific implementation steps are as follows:
[0035] A. First, weigh 29g of acrylamide and 1g of N,N-methylenebisacrylamide into the mixing container;
[0036] B. Then, add ultrapure water to the container, so that acrylamide and N,N-methylenebisacryla...
Embodiment 2
[0058] As another preferred embodiment of the present invention, the difference from the previous embodiment is that it is a polyacrylamide gel for the separation of small molecular weight proteins, which includes acrylamide, N, N-methylenebisacrylamide , ultrapure water, gel buffer, glycerol, ammonium persulfate solution and tetramethylethylenediamine, the gel buffer includes boric acid and Tris-HCl solution, the pH of the gel buffer is 7.0, and the concentration of Tris is 50-200mM, the concentration of boric acid is 50-200mM.
[0059] The present invention also provides a method for preparing polyacrylamide gel used for the separation of small molecular weight proteins, comprising the following steps:
[0060] Step 1: prepare 30% acrylamide stock solution, wherein the mass ratio of acrylamide to N,N-methylene bisacrylamide is 29:1, the specific implementation steps are as follows:
[0061] A. First, weigh 29g of acrylamide and 1g of N,N-methylenebisacrylamide into the mixi...
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