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Method and device for extracting polygonum capitatum root secretions

A technology of root exudates and extraction method, which is applied in sampling devices, measuring devices, analyzing materials, etc., can solve the problems of continuous cropping obstacles of Polygonum annua, decline in yield and quality of Polygonum angustifolia, etc.

Pending Publication Date: 2019-12-03
GUIYANG UNIV
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Problems solved by technology

[0005] Plant root exudates are one of the important factors leading to continuous cropping obstacles. Studies have shown that Polygonum cephalosporins have serious continuous cropping obstacles, which directly lead to the decline in yield and quality of Polygonum cephalum.

Method used

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  • Method and device for extracting polygonum capitatum root secretions

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Embodiment

[0027] Embodiment: a method for extracting root exudates of Polygonum cephalum, comprising the steps of:

[0028] 1) The Polygonum cephalum seeds are first germinated, and then germinated with a nutrient bowl after germination; the specific method is as follows:

[0029] a) Pad 3-4 layers of gauze at the bottom of the petri dish, cover the filter paper on the gauze, add water to soak the gauze and filter paper;

[0030] b) Sow the seeds on the filter paper of the petri dish, and then cover with a layer of filter paper for shading and moisturizing, and cultivate it at 23-28°C until it turns white;

[0031] 3) sow the dew-white seeds in step b) in a nutrient pot equipped with nutrient soil, cover with river sand for 3-5mm, then cover the nutrient pot with plastic wrap, and germinate at 23-28°C in the dark;

[0032] 4) After it germinates completely, remove the plastic wrap, water regularly, and transplant after 60 days.

[0033] 2) Select Polygonum cephalum seedlings in the sa...

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Abstract

The invention discloses a method and device for extracting polygonum capitatum root secretions. The method can be used for dynamically extracting the polygonum capitatum root secretions in vivo, has the characteristics of not damaging a plant root, avoiding bleeding sap in root secretions, and continuously extracting the root secretions of the same plant in different growth periods, and can meet the requirement of dynamically extracting the polygonum capitatum root secretions in different growth periods. The polygonum capitatum root secretions are dynamically extracted to analyze the components of the polygonum capitatum root secretions, and the method has important guiding significance for revealing the influence mechanism of the polygonum capitatum root secretions in different growth periods on the polygonum capitatum and overcoming the continuous cropping obstacles of the polygonum capitatum.

Description

technical field [0001] The invention belongs to the technical field of overcoming obstacles in traditional Chinese medicine continuous cropping, in particular to a method and a device for extracting root exudates of Polygonum cephalum. Background technique [0002] Polygonum capitatum (Polygonum capitatum Buch.-Ham.ex D.Don) belongs to the perennial herbaceous plant of the genus Polygonum, and is one of the famous Miao medicines. Polygonum is also one of the varieties in the "six major Miao medicines" that are focused on the development of traditional Chinese medicine modernization in Guizhou Province and the "seven major traditional Chinese medicine industry chains" that are cultivated and developed in Guizhou. For example, "Relinqing Granules" have the functions of clearing heat and dampness, detoxifying and relieving pain, harmonizing blood and dispelling blood stasis, diuresis and treating stranguria, and are widely used in the treatment of urinary system diseases. In a...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N1/10G01N1/34
CPCG01N33/0098G01N1/10G01N1/34
Inventor 赵许朋刘燕王继玥赵晓朋刘会黄杰城
Owner GUIYANG UNIV
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