Unlock instant, AI-driven research and patent intelligence for your innovation.

Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system

A compound amplification system and primer combination technology, applied in the field of forensic technology, can solve the problems of time-consuming, laborious, false negative, false positive, etc.

Inactive Publication Date: 2019-12-06
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional bloodstain identification methods are mainly biochemical and immunological analysis methods, that is, using hemoglobin and its derivatives in the blood for identification, but such methods are not only time-consuming and laborious, but also consume a lot of test materials, and are prone to false positive or false negative results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system
  • Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system
  • Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1, the preparation of the compound amplification system based on 6 genes

[0091] 1. Screening of specifically expressed genes

[0092] The inventors of the present invention obtained a large number of candidate genes specifically expressed in peripheral blood, menstrual blood, saliva, semen, and vaginal secretions by consulting the literature, comprehensively considered the sequence structure of each gene, and finally screened and obtained 4 specifically expressed genes (respectively) HBA gene, HBB gene, MMP7 gene, MMP10 gene) and two housekeeping genes (GAPDH gene and ACTB gene respectively).

[0093] 2. Preparation of multiple amplification system based on 6 genes

[0094] According to the nucleotide sequence of each gene and the length range of the amplified fragment (between 100bp-300bp), different amplified fragment lengths were reasonably selected to distinguish each gene, and then a multiple amplification system based on 6 genes was prepared. Specif...

Embodiment 2

[0143] Example 2. Application of multiple amplification system based on 6 genes in identifying whether the body fluid to be tested is non-blood, menstrual blood or peripheral blood

[0144] 1. Sample collection

[0145] The samples to be tested were 80 samples provided by 80 volunteers (aged 18-35) from Henan. 80 volunteers were unrelated individuals, and all of them gave informed consent.

[0146] The 20 samples numbered PB1-PB20 are all peripheral blood samples. Peripheral blood samples were obtained from venous blood drawn from the arms of some volunteers and stored at -80°C.

[0147] The 20 samples numbered MB1-MB20 are all menstrual blood samples. The 20 menstrual blood samples were collected by some volunteers with tampons 4 days before the menstrual cycle, dried at room temperature for 1 day, and stored at -80°C.

[0148] The 20 samples numbered SA1-SA20 are all saliva samples. The 20 saliva samples were obtained by collecting saliva from some volunteers in sterile...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and a primer combination used by the multiplex amplification system. The primer combination is composed of 12 DNA molecules shown in sequences 1 to 12 in a sequence table. By adopting the primer combination to construct the multiplex amplification system, whether body fluid to be detected is non-blood, menstrual blood or peripheral blood can be identified, and the accuracy is high. The multiplex amplification system provides an accurate scientific basis for defining case properties, determining crime sentences and the like, and has important application value.

Description

technical field [0001] The invention belongs to the field of forensic technology, and in particular relates to a compound amplification system for identifying whether a body fluid to be tested is non-blood, menstrual blood or peripheral blood and a primer combination used therefor. Background technique [0002] By identifying the tissue source of body fluid stains at the crime scene, the source of sample tissue and its real criminal activities can be inferred, thereby providing important information for case characterization and scene reconstruction, and important scientific evidence for forensic science. Common bodily fluids at the scene of the case include peripheral blood, menstrual blood, saliva, semen, and vaginal secretions. Among them, although both peripheral blood and menstrual blood contain a large number of red blood cells, compared with peripheral blood, menstrual blood also contains a large number of mixtures such as endometrial fragments, cervical mucus, and va...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6876
CPCC12Q1/6876C12Q2600/158
Inventor 赵一霞孙启凡季安全胡胜
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY