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A kind of preparation method of human umbilical cord mesenchymal stem cells

A technology of mesenchymal stem cells and human umbilical cord, applied in the field of stem cells, can solve problems such as the inability of cells to adhere to the wall

Active Publication Date: 2021-09-03
福建省海西细胞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the enzyme digestion method can quickly isolate umbilical cord mesenchymal stem cells, the enzyme system may degrade the outer membrane proteins of the cells, and even cause the cells to fail to adhere to the wall

Method used

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  • A kind of preparation method of human umbilical cord mesenchymal stem cells
  • A kind of preparation method of human umbilical cord mesenchymal stem cells
  • A kind of preparation method of human umbilical cord mesenchymal stem cells

Examples

Experimental program
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Embodiment 1

[0040] A method for preparing human umbilical cord mesenchymal stem cells, comprising the following steps:

[0041] (1) Collection: The umbilical cords from the 900th Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army were selected for normal full-term delivery. After signing the informed consent with the puerpera and their family members, the umbilical cord was routinely cut off after the fetus was delivered, and the umbilical cord was automatically cut with iodophor gauze. Wipe quickly from the broken end of the umbilical cord to the broken end of the placenta, disinfect twice and remove blood amniotic fluid and meconium, and wipe once with gauze containing normal saline to remove povidone iodine, drain the cord blood from the cut and wipe it with sterile gauze to remove all the cord blood . Clamp the head and tail of the umbilical cord with a hemostat, and then ligate it with sterile silk near the hemostat. Put the ligated umbilical cord ...

Embodiment 2

[0075] A method for preparing human umbilical cord mesenchymal stem cells, comprising the following steps:

[0076] (1) Collection: 3 cases of umbilical cords (marked as: umbilical cord 01, umbilical cord 02, and umbilical cord 03) from the 900th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army were selected, and signed informed consent with the puerpera and their families Book, after the fetus is delivered, the umbilical cord is cut off routinely, quickly wiped with iodophor gauze from the broken end of the umbilical cord to the broken end of the placenta, disinfected twice and cleared the blood amniotic fluid and meconium, and wiped once with gauze containing normal saline to remove the iodophor, The umbilical cord blood was drained and wiped with sterile gauze to remove the umbilical cord blood. Clamp the head and tail of the umbilical cord with a hemostat, and then ligate it with sterile silk near the hemostat. Put the ligated umbilica...

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Abstract

The invention discloses a method for preparing human umbilical cord mesenchymal stem cells, belonging to the field of stem cell preparation. The preparation method of the present invention includes the whole process of collection, separation, liquid replacement, subculture, freezing storage, detection, programmed cooling and storage. Umbilical cord mesenchymal stem cells are extracted from human umbilical cord, and prepared by two-step digestion with two enzymes. The digested cells and the remaining tissue after digestion are separated and cultured, then subcultured and mixed together for culture. No need to add animal serum during the extraction and culture process , the preparation process does not need to add antibiotics, the primary separation does not need to be filtered, and the two culture methods are carried out at the same time, which not only improves the success rate but also can obtain a large number of stable cells in a short period of time. The method has a short primary extraction and digestion time, avoids the influence of type I collagenase and dispase on the cells after long-term digestion, and simultaneously avoids the loss of cell numbers after primary digestion and filtration of cells. The entire culture period is short, and umbilical cord mesenchymal stem cells can be obtained efficiently and stably.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to a preparation method of human umbilical cord mesenchymal stem cells. Background technique [0002] Stem cells are a type of cells with high self-renewal and multi-directional differentiation potential, which can be cultured, expanded, and directional induced to differentiate into various cells in vivo. Stem cells can be divided into embryonic stem cells and adult stem cells according to the order in which they appear during individual development. Adult stem cells exist in tissues such as umbilical cord, bone marrow, fat, blood, and dental pulp. Research on adult stem cells and plasticity and regenerative medicine have become one of the hot spots in the field of biomedicine in recent years. They are widely used in the treatment of bone-related diseases and nerve cell repair. , angiogenesis, immune regulation, cell replacement therapy, anti-aging and tissue enginee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 沈少敏聂雅婷吕小婷何锎钋
Owner 福建省海西细胞生物工程有限公司