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Composition, method and system for determination of digital polymerase chain reaction (PCR)

A technology of polymerase and chain reaction, applied in the field of digital polymerase chain reaction, which can solve the problems of inability to achieve amplification, low efficiency of PCR reaction, and limitation of the application of digital PCR technology, and achieve high reaction efficiency and good stability

Inactive Publication Date: 2019-12-10
BEIJING ZHIYU BIOTECH LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although digital PCR technology has the above advantages, however, there are still many reagent systems, and their PCR reaction efficiency in microdroplets is low and cannot achieve ideal amplification, which limits the application of digital PCR technology.

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  • Composition, method and system for determination of digital polymerase chain reaction (PCR)
  • Composition, method and system for determination of digital polymerase chain reaction (PCR)

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Embodiment Construction

[0038] In the prior art, the research on liquid droplets in digital PCR reaction technology mainly focuses on how to improve the stability of liquid droplets, and few people improve the efficiency of PCR reaction through the design of liquid droplets.

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Abstract

The invention discloses a composition, method and system for determination of a digital polymerase chain reaction (PCR). The composition comprises a water phase for generating water-in-oil liquid droplets, and the water phase contains water and fibroin nanofibers dissolved in the water. The composition particularly is an emulsion formed by the water phase and an oil phase which are mixed, whereinthe oil phase constitutes a continuous phase, and the water phase forms the multiple liquid droplets scattered in the continuous phase. The water phase contains the fibroin nanofibers, the water phasecan enter the liquid droplet interface to participate in liquid droplet formation, the liquid droplets formed by the water phase have the significantly higher reaction efficiency during the PCR, andthe stability of the liquid droplets is improved. The method for determination of the digital PCR has the high reaction efficiency and is suitable for determination of various target nucleic acids.

Description

technical field [0001] The invention relates to the technical field of digital polymerase chain reaction (PCR), in particular to a composition, a method and a system suitable for liquid drop polymerase chain reaction determination. Background technique [0002] Polymerase chain reaction (PCR) technology is one of the most important tools in modern biology. It is widely used in medical diagnosis, personalized medicine, food inspection, genetically modified organism detection, pathogen identification, immune analysis, forensic science, etc. As the latest generation of PCR technology, digital PCR (dPCR) based on the development of microfluidic technology has smaller reaction volume, faster reaction speed, lower system noise and higher sensitivity than traditional qPCR. [0003] dPCR technology, through the water-in-oil structure, encapsulates a single DNA molecule into a separate droplet, using the inertness of oil to achieve mutual isolation between DNA molecules, and each DNA...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2563/159
Inventor 李昂
Owner BEIJING ZHIYU BIOTECH LTD
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