Detection kit for clostridium cadaveris from goat source

A detection kit and detection reagent technology, applied in the field of detection kits for Clostridium cadavericum from sheep, can solve the problems of no detection of PCR primers or kits, etc., and achieve high-sensitivity effects

Inactive Publication Date: 2019-12-10
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Clostridium cadavericum needs to survive in the host's immune system after invading the host, and will undergo adaptive evolution, resulting in certain differences in the genomes of Clostridium cadavericum in different types of host organisms, so that according to genbank et al. Routine PCR primer design with genomic databases may not necessarily detect Clostridium cadavericum isolated from a variety of different animals
At present, there is no report of Clostridium cadavericum from sheep (with sheep as the host), so there is no PCR primer or kit for detecting Clostridium cadavericum from sheep

Method used

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  • Detection kit for clostridium cadaveris from goat source
  • Detection kit for clostridium cadaveris from goat source
  • Detection kit for clostridium cadaveris from goat source

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1 Kit of the present invention

[0028] 1. Composition of the kit

[0029] This kit is a triple PCR detection kit, which consists of the following parts:

[0030] Triple PCR primers, PCR master mix (1.1×T3 Super PCR Mix).

[0031] Primer sequences are shown in Table 1.

[0032] Table 1 Triple PCR primer information

[0033]

[0034] 2. How to use the kit

[0035] Mix 18 μL of 1.1×T3 Super PCR Mix, 1 μL of Clostridium cadavericum DNA template, and 1 μL each of the upstream and downstream primers of the three pairs of primers, and then detect by agarose gel electrophoresis after PCR.

[0036] PCR conditions were: pre-denaturation at 98°C for 2 min; denaturation at 98°C for 10 s, annealing at 59°C for 15 s, extension at 72°C for 30 s, a total of 35 cycles; extension at 72°C for 2 min.

[0037] The beneficial effects of the kit of the present invention will be further introduced in the form of experimental examples below.

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Abstract

The invention belongs to the field of PCR (polymerase chain reaction) detection kits, and particularly relates to a detection kit for clostridium cadaveris from goat source. The kit is characterized by comprising primer pairs represented as SEQ ID NO.1-2, SEQ ID NO.3-4 and / or SEQ ID NO.5-6; and the primers are non-interfering and high-sensitivity and high-specificity detection can be completed bythe kit. The kit fills up the blank of a detection reagent for the clostridium cadaveris from goat source and has good application prospects.

Description

technical field [0001] The invention belongs to the field of PCR detection kits, in particular to a detection kit for Clostridium spp. Background technique [0002] Clostridium cadaveris (also known as Clostridium cadaveris) is one of the most common bacteria found in decomposing corpses, but is extremely rare in living humans. The bacterium is widely distributed in soil, seabed sediment and human feces. The bacterium is an anaerobic Gram-positive bacillus with perinatal flagellar movement and spores that are oval and terminal. Clostridium cadavericus is a member of group IV of the genus Clostridium in the family Bacillus, which also includes Clostridium lentifera, Clostridium putrefaciens, Clostridioides hydra, Clostridium tetani, and Clostridium puterogenes. [0003] Clostridium cadavericus is a pathogenic bacterium that can be detected in clinical samples such as wound infections, abscesses, and blood samples. It is necessary for people to take preventive and monitoring...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/145
CPCC12Q1/689C12Q1/686C12Q2537/143
Inventor 王利豆朋朋
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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