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Production method of efficient phosphorus-dissolving epicoccum sorghinum with characteristic of resisting heavy metals

A production method and heavy metal technology, applied in the field of environmental microorganisms, can solve problems such as low utilization of soil phosphorus, and achieve the effects of promoting the growth of local vegetation, reducing the use of chemical fertilizers, and improving soil fertility

Inactive Publication Date: 2019-12-17
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of low utilization rate of phosphorus in soil, the object of the present invention is to provide a production method of a high-efficiency E. phospholyticum with the characteristics of tolerance to heavy metals

Method used

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  • Production method of efficient phosphorus-dissolving epicoccum sorghinum with characteristic of resisting heavy metals
  • Production method of efficient phosphorus-dissolving epicoccum sorghinum with characteristic of resisting heavy metals

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Experimental program
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Effect test

Embodiment 1

[0034] A preparation method of a high-efficiency E. phospholyticum strain with the characteristic of tolerance to heavy metals, comprising the following steps:

[0035] (1) Extract the mixed strain from the soil: first, mix the copper tailings soil and sterile water according to the volume ratio of 1:10, then shake and cultivate, shake and cultivate at 30°C for 24 hours, centrifuge and discard the supernatant;

[0036] (2) Add pb, Zn, Cu, Cd, Cr respectively to the precipitate in proportion to obtain a heavy metal sterile mixed solution, carry out shaking culture to obtain a culture solution, add the volume ratio of the heavy metal-containing sterile culture solution to the precipitate: : heavy metal sterile culture solution = 1:10, cultivated at a constant temperature of 30°C for 24h.

[0037] (3) Draw the culture solution obtained in step (2), add the heavy metal sterile mixed solution in step (2) again in an iso-concentration gradient, and carry out shaking culture;

[003...

Embodiment 2

[0052] The difference between this embodiment 2 and embodiment 1 is:

[0053] Described organophosphate medium composition comprises: 10g / L glucose, 0.5g / L (NH 4 ) 2 SO 4 , 0.3g / L NaCl, 0.71g / L K 2 SO 4 , 0.3g / L MgSO 4 ·7H 2 O, 0.0204g / L MnSO 4 , 0.03g / L FeSO 4 , 1.0g / L CaCO 3 , 0.5 g lecithin (7 g Tween 80), 20.0 g agar, pH=6.5-7.5, sterilized at 121° C. for 30 min.

[0054] Described inorganic phosphorus medium composition comprises: 10g / L glucose, 0.5g / L (NH 4 ) 2 SO 4 , 0.3g / L NaCl, 0.71g / L K 2 SO 4 , 0.3g / L MgSO 4 ·7H 2 O, 0.0204g / L MnSO 4 , 0.03g / L FeSO 4 , 5.0g / L Ca 3 (PO 4 ) 2 , Agar 20.0g, pH=6.5-7.5, sterilized at 121°C for 30min.

Embodiment 3

[0056] Screening and Identification of Phosphorus Solubilizing Fungi:

[0057] The Eperococcus strain of this embodiment is isolated and screened from the surface 5cm-20cm soil samples of the copper tailings area. Screening is carried out as follows: the collected soil is brought back to the laboratory immediately, placed in a refrigerator at -20°C for preservation, 10g of the sieved soil is put in 100ml sterile water with glass beads to make a soil suspension, The concentration of this suspension is 10 -1 , put the suspension on a constant temperature oscillator and shake it fully at 150r / min for 30min, take it out and centrifuge and discard the supernatant, take 10ml from the sample bacterial suspension and add it to 90ml sterile water to make the bacterial suspension Concentration is 10 -2 , and then perform 10-fold serial dilution in this way until the concentration of the diluent is 10 -4 until. take 10 -4 Concentration of bacterial suspension 1ml, placed on the sele...

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Abstract

The invention belongs to the field of environmental microorganisms, and particularly relates to a production method of efficient phosphorus-dissolving epicoccum sorghinum with the characteristic of resisting heavy metals. Through classification, the epicoccum sorghinum is identified. The production method includes the steps that copper tailing soil and sterile water are cultured in a mixed mode, and a heavy metal sterile mixed solution with the concentration suitable for the growth of thalluses is screened; repeated culture is conducted for six times; fourth-time culture liquid, fifth-time culture liquid and sixth-time culture liquid are sucked and added into culture mediums containing organic phosphorus and inorganic phosphorus for plate spread culture, culture dishes with a large numberof bacterial colonies are selected, plate streaking culture is conducted in the culture mediums containing the organic phosphorus and the inorganic phosphorus, and the bacterial colonies with obviousphosphorus-dissolving rings are screened; and culture purification results are subjected to shake cultivation, and finally screening purification is conducted to obtain phosphorus-dissolving bacterialstrains resisting the heavy metals. Efficient phosphorus-dissolving funguses resisting the heavy metals are screened from contaminated land, the soil phosphorus supply and demand contradiction can beadjusted, reclamation land soil in a mining area is improved, vegetation growth is promoted, the utilization ratio of heavy metal contaminated plowland soil phosphorus by crops is increased, and useof fertilizer is reduced.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a preparation method of a high-efficiency phospholyticum strain with the characteristic of tolerance to heavy metals. Background technique [0002] Phosphorus is one of the essential nutrients for plants, and 74% of the cultivated land in my country is deficient in phosphorus. More than 95% of the phosphorus in the soil is in the invalid form, that is, insoluble phosphorus compounds, which are difficult for plants to directly absorb and utilize. The crop utilization rate of the applied phosphorus fertilizer is 5%-25%, most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ And other metal ions combine to form insoluble phosphate. Therefore, improving the utilization rate of phosphorus has always been a concern of agronomists and ecologists. There are many factors affecting the utilization efficiency of soil phosphorus, among which microorganisms h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 沈章军李士玉王倩倩季涛涛徐德聪李玲玲王晶晶
Owner HEFEI NORMAL UNIV
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