A kind of nanometer diagnosis and treatment agent and its preparation method, application
A nanometer and nanoparticle technology, applied in the field of biomedical materials, can solve the problems that the particles are not easy to decompose, and it is difficult to treat tumors.
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Embodiment 1
[0056] Example 1: Synthesis of nano-therapeutic agent (MnS@BSA)
[0057] Add 1 mL of 10 mg / mL bovine serum albumin (BSA) solution to 40 mL of water, then add 400 µL of 0.5 M sodium sulfide solution to mix, and adjust the pH value to 7.0-8.0 (preferably 7.4) with acidic solution ), the system was vacuumed and protected under nitrogen gas, stirred at room temperature and slowly added 4 mL of manganese nitrate solution with a concentration of 0.05 M to obtain a pink solution, which was centrifuged, washed with water and freeze-dried to obtain bovine serum albumin A nano-therapeutic agent doped with 𝛽-phase manganese sulfide nanoparticles.
[0058] figure 2 It is the TEM image of the synthetic nano-therapeutic agent (MnS@BSA in the metastable phase); image 3 It is the XRD pattern of the synthetic nano-therapeutic agent (MnS@BSA); Figure 4 It is the SEM element distribution map of the synthetic nano-therapeutic agent (MnS@BSA); figure 1 , figure 2 and image 3 Descriptio...
Embodiment 2
[0059] Example 2: Morphology control of synthetic nano-therapeutic agent (MnS@BSA) under different ratio conditions
[0060] Add 1 mL of bovine serum albumin solutions with concentrations of 2.5, 5, and 10 mg / mL to 40 mL of water, then add 400 µL of 0.5 M sodium sulfide solution to mix, and adjust the pH value to 7.4-7.8 with acidic solution , the system was evacuated and protected under nitrogen gas, stirred at room temperature and slowly added 2 mL of manganese nitrate solution with a concentration of 0.05 M to obtain a flesh-pink solution, which was washed by centrifugation and freeze-dried to obtain a nano-medicine.
[0061] Figure 5 The middle represents the TEM images of the morphology adjustment of the synthetic nano-therapeutic agent at different ratios. The results show that the particle size of the nano-therapeutic agent becomes smaller and smaller with the increase of bovine serum albumin (BSA). It shows that adjusting the ratio of protein can adjust the particle ...
Embodiment 3
[0062] Example 3: Evaluation of degradation performance and release effect of synthetic nano-therapeutic agent (MnS@BSA)
[0063] The synthesized nano-diagnostic agent (MnS@BSA) was added to the buffer solution with pH=6.8 and pH=7.4 respectively, and the sampling point was placed on the TEM copper grid at 5, 30, and 60 min to observe the degradation process; 200 uL of the synthesized nano The diagnostic agent (MnS@BSA) was added into a dialysis bag with a molecular weight of 3500 Da, sealed and put into a buffer solution with pH=6.8 and pH=7.4, and taken out at 0, 0.5, 1, 2, 4, 8, 12, and 24 h 2 mL of solution, and replenish 2 mL of new buffer solution at the same time, analyze the content of element manganese in samples at different time points by ion inductive coupling (ICP), and calculate the release amount of manganese element; BSA) was added to a dialysis bag with a molecular weight of 3500 Da, sealed and put into a 0-degree ice-water bath buffer solution with pH=6.8 and...
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