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Nanometer diagnosis and treatment agent and preparation method and application thereof

A nanometer and nanoparticle technology, applied in the field of biomedical materials, can solve problems such as difficult to treat tumors, particles are not easy to decompose, etc., and achieve the effect of overcoming limitations and efficient tumor treatment

Active Publication Date: 2019-12-20
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

b class="d_n">[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a nano-diagnostic and therapeutic agent and its preparation method and application, aiming at solving the difficulty of decomposing the existing manganese sulfide diagnostic and therapeutic agent particles, Technical issues that are difficult to use in tumor therapy

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  • Nanometer diagnosis and treatment agent and preparation method and application thereof
  • Nanometer diagnosis and treatment agent and preparation method and application thereof
  • Nanometer diagnosis and treatment agent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1: Synthesis of nano-therapeutic agent (MnS@BSA)

[0057] Add 1 mL of 10 mg / mL bovine serum albumin (BSA) solution to 40 mL of water, then add 400 µL of 0.5 M sodium sulfide solution to mix, and adjust the pH value to 7.0-8.0 with acidic solution (preferably After 7.4), vacuumize the system and protect it under nitrogen gas. Stir at room temperature and slowly add 4 mL of manganese nitrate solution with a concentration of 0.05 M to obtain a flesh-pink solution. After centrifugal washing and freeze-drying, bovine serum white is obtained. A nano-therapeutic agent in which proteins and 𝛽-phase manganese sulfide nanoparticles are doped with each other.

[0058] figure 2 It is the TEM image of the synthetic nano-therapeutic agent (MnS@BSA in the metastable phase); image 3 It is the XRD pattern of the synthetic nano-therapeutic agent (MnS@BSA); Figure 4 It is the SEM element distribution map of the synthetic nano-therapeutic agent (MnS@BSA); figure 1 , figur...

Embodiment 2

[0059] Example 2: Morphology control of synthetic nano-therapeutic agent (MnS@BSA) under different ratio conditions

[0060] Add 1 mL of bovine serum albumin solution with a concentration of 2.5, 5, and 10 mg / mL to 40 mL of water, then add 400 µL of 0.5 M sodium sulfide solution to mix, and adjust the pH value to 7.4-7.8 with an acidic solution. Vacuumize the system and protect it under nitrogen gas. Stir at room temperature and slowly add 2 mL of manganese nitrate solution with a concentration of 0.05 M to obtain a flesh-pink solution. After centrifugation, washing with water and freeze-drying, the nano-therapeutic agent is obtained.

[0061] Figure 5 The middle represents the TEM images of the morphology adjustment of the synthetic nano-therapeutic agent at different ratios. The results show that the particle size of the nano-therapeutic agent becomes smaller and smaller with the increase of bovine serum albumin (BSA). It shows that adjusting the ratio of protein can adjus...

Embodiment 3

[0062] Example 3: Evaluation of degradation performance and release effect of synthetic nano-therapeutic agent (MnS@BSA)

[0063] The synthesized nano-diagnostic agent (MnS@BSA) was added to the buffer solution with pH=6.8 and pH=7.4 respectively, and the sampling point was placed on the TEM copper grid at 5, 30, and 60 minutes to observe the degradation process; 200 uL of the synthesized nano-diagnostic agent Add the agent (MnS@BSA) into a dialysis bag with a molecular weight of 3500 Da, seal it and put it into a buffer solution with pH=6.8 and pH=7.4, take out 2 mL at 0, 0.5, 1, 2, 4, 8, 12, and 24 hours At the same time, add 2 mL of new buffer solution, analyze the content of manganese in samples at different time points by ion inductive coupling (ICP), and calculate the release of manganese; 200 uL of synthetic nano-medicine (MnS@BSA) Put it into a dialysis bag with a molecular weight of 3500 Da, seal it and put it into a 0-degree ice-water bath buffer solution with pH=6.8...

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Abstract

The invention provides a nanometer diagnosis and treatment agent and a preparation method and application thereof. The nanometer diagnosis and treatment agent comprises protein and beta-phase manganese sulfide nanometer particles mutually doped with the protein. The nanometer diagnosis and treatment agent is easy to degrade, has a pH-responsive characteristic, and can release more hydrogen sulfidegas at tumor locations, and the treatment effect of the hydrogen sulfide gas is achieved; and manganese ions released from manganese sulfide have the characteristic of a fenton-like reaction under the tumor environment, a hydrogen peroxide solution at the tumor locations can be decomposed to produce hydroxyl free radicals having strong oxidizing properties, and the hydroxyl free radicals are usedfor chemical kinetics treatment of tumors, so that the limitation of single gas treatment or single chemical kinetics treatment of the tumors is overcome, and high-efficiency tumor treatment effectsare achieved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a nanometer diagnosis and treatment agent and its preparation method and application. Background technique [0002] The treatment of malignant tumors is one of the biggest problems facing public health. Designing and manufacturing new nano-therapeutic agents is a hotspot in the diagnosis and treatment of tumors. Among them, manganese-based nanomaterials, including manganese oxide, manganese dioxide, manganese sulfide, manganese tetraoxide, manganese carbonate, manganese phosphate, etc., are widely used because of their degradability, multiple responsiveness and good biocompatibility. reports. There are literature reports that grow manganese dioxide nanospheres on the surface of silicon dioxide. The manganese dioxide in the nanospheres releases manganese ions under the stimulation of the tumor microenvironment, which can be used for magnetic resonance imaging. [0...

Claims

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Application Information

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IPC IPC(8): A61K33/32A61K33/04A61K9/51A61K47/42A61P35/00A61K49/14A61K49/18
CPCA61K33/32A61K33/04A61K9/5169A61P35/00A61K49/143A61K49/1869
Inventor 黄鹏贺婷林静覃夏灵
Owner SHENZHEN UNIV
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