Method of constructing fluorescence sensor in spectral analysis for detecting prostate-specific antigen and application thereof
A prostate-specific and fluorescent sensor technology, applied in the field of spectral analysis, can solve the problems of complex preparation process, poor reproducibility, unable to completely eliminate the background interference of biological samples, etc., and achieve the effects of high accuracy and sensitivity, high sensitivity and specificity
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Embodiment 1
[0038] A construction method of a fluorescence sensor in the spectral analysis for detecting prostate specific antigen, the construction method comprises the steps:
[0039] (1) Preparation of ZGO:Mo / PSA-A solution: Synthesis and modification of Mo-doped long afterglow nanorods: 2mmol Zn(NO 3 ) 2 , 0.005mmol Na 2 MoO 4 , with 300 μL concentrated HNO 3 Mix with vigorous stirring, and add 11 mL of ultrapure water to form a colorless and transparent solution. Weigh 1.3g GeO 2 And 1.5g NaOH was dissolved in 21mL ultrapure water, stirring continuously for 7h until the solution was clear to obtain Na 2 GeO 3 solution. The prepared 1.5 mL of Na 2 GeO 3 The solution was added dropwise to the above solution, and the pH value of the solution was adjusted to 7 by adding ammonia water. The solution gradually changed from clarification to white turbidity. After stirring continuously and rapidly at room temperature for 1 h, it was transferred to a polytetrafluoroethylene hydrotherm...
Embodiment 2
[0043] A construction method of a fluorescence sensor in the spectral analysis for detecting prostate specific antigen, the construction method comprises the steps:
[0044] (1) Preparation of ZGO:Mo / PSA-A solution: Synthesis and modification of Mo-doped long afterglow nanorods: 2.1 mmol Zn(NO 3 ) 2 , 0.0055mmol Na 2 MoO 4 , with 350 μL concentrated HNO 3 Mix with vigorous stirring, and add 11.5 mL of ultrapure water to form a colorless and transparent solution. Weigh 1.45g GeO 2 And 1.65g NaOH was dissolved in 21mL ultrapure water and stirred continuously for 7h until the solution was clear to obtain Na 2 GeO 3 solution. The prepared 1.75 mL of Na 2 GeO 3 The solution was added dropwise to the above solution, the pH value of the solution was adjusted to 8 by adding ammonia water, the solution gradually changed from clarification to white turbidity, and was continuously and rapidly stirred at room temperature for 1 h, then transferred to a polytetrafluoroethylene hydr...
Embodiment 3
[0048] A method for constructing a fluorescence sensor in spectral analysis for detecting prostate-specific antigen, the construction method comprising the following steps: (1) preparation of ZGO:Mo / PSA-A solution: synthesis and modification of Mo-doped long afterglow nanorods : 2.2mmolZn(NO 3 ) 2 , 0.006mmol Na 2 MoO 4 , with 400 μL concentrated HNO 3 Mix with vigorous stirring, and add 12 mL of ultrapure water to form a colorless and transparent solution. Weigh 1.6g GeO 2 And 1.8g NaOH was dissolved in 21mL ultrapure water and stirred continuously for 7h until the solution was clear to obtain Na 2 GeO 3 solution. The prepared 2 mL of Na 2 GeO 3The solution was added dropwise to the above solution, and the pH value of the solution was adjusted to 10 by adding ammonia water. The solution gradually changed from clarification to white turbidity. After stirring continuously for 1 h at room temperature, it was transferred to a polytetrafluoroethylene hydrothermal reaction...
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