Full-automatic cell culturing system and method with high adaptability

Abstract

The invention relates to a full-automatic cell culturing system and method with high adaptability. The system comprises an isolation unit, a culturing unit, a centrifugal transfection unit and a gathering unit, wherein the isolation unit is used for separating and cleaning a batch of cells and conducting inoculating; the culturing unit is used for performing culturing preparation on inoculated cells; the centrifugal transfection unit is used for performing virus centrifugal transfection and cell harvesting on the cells obtained through culturing preparation; and the gathering unit is used fordetecting the obtained cells and classifying and transferring out the obtained cells. According to the full-automatic cell culturing system with the high adaptability disclosed by the invention, through automatically controlling a transferring unit, the cells are transferred into the isolation unit, the culturing unit, the centrifugal transfection unit and the gathering unit, full-automatic culturing of the cells is realized, so that the culturing efficiency is improved; and the system and method have high adaptability to adjustment upgrade of a production process requires in a batch production step; and moreover, basic data for the cultured cells can be detected, and a qualification ratio of the cells is strictly controlled.

Description

technical field [0001] The invention relates to a cell preparation system, more specifically to a highly adaptable automatic cell culture system and a method thereof. Background technique [0002] The clinical efficacy of cell therapy has been verified. Domestic and foreign policies strongly support the development of related industries. The industry has ushered in a development trend, and there is a huge demand for large-scale cell preparation. Therefore, cell culture processes are required to obtain large-scale cells. , Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. No matter for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a large-scale cloning of cells. Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essenti...

AI Technical Summary

Problems solved by technology

[0003] At this stage, cell culture at home and abroad is still mainly manual production. When expanding production capacity on a large scale, a large number of personnel costs, a large number of personnel training costs, large-area high-cleanliness facility construction costs, and high certification costs for new facilities, etc., make Industrial investment is unaffordable

At the same time, the quality instability and inhomogeneity brought about by manual preparation make it difficult to meet the high-quality and safety requirements of cell therapy. In the manual preparation mode, the flow of personnel also brings a greater risk of leakage of the company's core intellectual property rights.

In addition, the existing manually operated semi-automated equipment on the market is aimed at partial process steps in the whole production process, and can only produce and prepare for a single patient at the same time, which brings great benefits to realize the scale of industrialized cell production and effectively reduce the cost of preparation and production. limited benefit

The barriers established by foreign manufacturers in consumables and other aspects have brought extremely high cost of use to end-patient users

At the same time, existing semi-automated equipment is usually customized and developed based on a specific production process, which is difficult to meet the adaptability and scalability requirements of different cell therapy programs and different production processes.

The quality and quality control of large-scale production in the later stage, the existing semi-automatic equipment is difficult to provide systematic production and quality control data

Although the production efficiency has been improved, the existing semi-automatic equipment still requires a lot of manual operation, and still requires the construction of a high-level clean room environment

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