Bacterial strain for preparing trehalose syrup by using soluble starch and application thereof

Abstract

The invention relates to a bacterial strain for preparing trehalose syrup by using soluble starch and application thereof, and can effectively solve the problems when soluble starch and bacterial strain HKS-D-3 are utilized for preparing low-cost and good-quality trehalose syrup. The bacterial strain HKS-D-3 for preparing trehalose syrup by utilizing soluble starch is classified and named as Bacillus sp., and is preserved in China General Microbiological Culture Collection Center, wherein the preservation number is CGMCC No.13052, the preservation date is September 28, 2016, the depository authority is China General Microbiological Culture Collection Center, and the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The strain HKS-D-3 is applied to the preparation of the trehalose syrup by utilizing the soluble starch. The preparation process is pollution-free, the product quality is good, the method is simple, the raw material cost is low, the liquidtrehalose syrup can be applied to a microorganism culture medium and can effectively promote growth of microorganisms and growth of hyphae, the product performance is improved, and the product qualityis good.

Description

technical field [0001] The invention relates to microorganisms, in particular to a bacterial strain which uses soluble starch to prepare trehalose syrup and its application. Background technique [0002] Trehalose exists widely in nature (such as microorganisms, bacteria, fungi, yeasts, insects, invertebrates and lower animals and plants). Disaccharides, with their stable chemical structure and special physical properties, make them able to maintain cell activity, prevent food deterioration, and improve food quality (Wang Yiwen, 2019), and are widely used in food, medicine, and light industry (Li Gaoxiang, 2015) The extensive and unique biological functions of trehalose have attracted widespread attention and triggered a worldwide research and development boom. However, the high production cost and high price of trehalose limit the application. The high cost of trehalose is mainly caused by the separation and purification of trehalose. The difficulty cost is high. There ar...

AI Technical Summary

Problems solved by technology

[0002] Trehalose exists widely in nature (such as microorganisms, bacteria, fungi, yeasts, insects, invertebrates and lower animals and plants). Disaccharides, with their stable chemical structure and special physical properties, make them able to maintain cell activity, prevent food deterioration, and improve food quality (Wang Yiwen, 2019), and are widely used in food, medicine, and light industry (Li Gaoxiang, 2015) The extensive and unique biological functions of trehalose have attracted widespread attention and triggered a worldwide research and development boom. However, the high production cost and high price of trehalose limit the application. The high cost of trehalose is mainly caused by the separation and purification of trehalose. high cost of difficulty

There are five synthetic pathways of trehalose in different organisms, which is a typical stress metabolite (Gong Tao, 2016). Its preparation methods include synthesis, fermentation, extraction, enzymatic conversion and genetic engineering ( Xie Ding, 2013), there are currently three main methods for preparing trehalose in the laboratory and production: one is to extract from yeast cells, but the extraction process is complicated, separation and extraction are difficult, and the cost is high; the other is to use microbial fermentation to produce trehalose from fermentation Trehalose is extracted from the fermentation liquid, which is difficult to extract due to the complex components of the fermentation liquid; the third is to use microbial fermentation to extract related trehalose synthases, and use enzyme transglycosylation to synthesize trehalose enzymatically. One of the most promising production methods, its appearance has laid a reliable foundation for the large-scale industrial production of trehalose

Our country is rich in starch resources with wide sources and low price. It is considered as the best raw material for the production of trehalose. Enzymatic production of trehalose with starch as a substrate is of great significance, but there has been no public report so far.