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Areca nut necrotic shuttle spot virus and detection method thereof

A detection method and virus technology, applied in the field of microorganisms, can solve the problems of no nucleic acid, detection methods, etc., and achieve great value

Active Publication Date: 2020-01-03
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports of this genus of viruses at home and abroad, and there is no nucleic acid detection method for this genus of viruses

Method used

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  • Areca nut necrotic shuttle spot virus and detection method thereof
  • Areca nut necrotic shuttle spot virus and detection method thereof
  • Areca nut necrotic shuttle spot virus and detection method thereof

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Embodiment Construction

[0031] The present invention will be further described below in combination with specific embodiments, so as to better understand the present invention.

[0032] The virus of the present invention is isolated from betel nut leaves, and the virus is named as arecapalm necrotic spindle-spot virus (Arecapalm necrotic spindle-spot virus) ANSSV-Baoting, which was preserved in the China Center for Type Culture Collection on June 17, 2018, with a deposit number It is CCTCC NO: V201828. The full genome sequence of the virus has been uploaded to the National Center for Biotechnology Information under the number MH330686.

[0033] Potatoviridae is the second largest virus family after Geminiviridae. Most viruses in this family have a wide range of hosts and often bring serious harm to agricultural production. According to the classification system revised by the International Committee Taxonomy of Viruses in 2017, the family includes 8 genera. The virus of the present invention is clo...

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Abstract

The invention belongs to the technical field of biology, and provides an areca nut necrotic shuttle spot virus. The virus is named areca nut necrotic shuttle spot virus ANSSV-Baoting, and the preservation number is CCTCC NO: V201828. The invention further provides a method for detecting the virus. The method includes three steps that a primer sequence required for nucleic acid detection is established, detection reaction types are established, and a detection reaction system and reaction conditions are established. The virus isolated from the leaves of areca nut is significantly different fromreported viruses of potato Y potyvirus and is a novel potyvirus of the potato Y potyvirus, a research basis is provided for studying areca nut necrotic spot shuttle disease, and the virus is of greatvalue in scientific research and cash crop protection; and in addition, according to the method for detecting the virus, the virus can be accurately and quickly detected, the efficient and reliable effects are realized, and the method is of great value in scientific research and cash crop protection.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a betel nut necrotic fustridium virus and a detection method thereof. Background technique [0002] Betel nut is the second largest tropical economic crop next to rubber in Hainan. It is called "green gold". The planting area is nearly 1.4 million mu, and its output accounts for 95% of the country. This is a living thing, and its pest control has always been one of the most concerned issues of the government, farmers and scientific research institutions, and plant virus diseases are currently the most harmful and difficult to control among diseases. In a recent survey, it was found that in Ding'an, Wanning, Baoting and other places, the incidence of betel nut necrotic fusiform virus disease was over 30%. The virus isolated from betel nut leaves was cloned and sequenced and entered the National Center for Biotechnology Information In comparison, it was found that it was signific...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N15/11C12Q1/70C12Q1/686C12R1/94
CPCC12N7/00C12Q1/701C12Q1/686C12Q2531/113C12Q2565/125Y02A50/30
Inventor 崔红光杨柯
Owner HAINAN UNIVERSITY
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