Magnetic particle luminescence micro-fluidic chip for multi-marker detection and detection device
A microfluidic chip, multi-marker technology, applied in measurement devices, fluid controllers, laboratory containers, etc., can solve the problems of reducing the accuracy of test results, easy cross-influence of magnetic particles, etc., to avoid mutual influence. Cross-influence, the effect of improving accuracy
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Embodiment 1
[0025] refer to Figure 1 to Figure 4 , the first embodiment provides a magnetic particle luminescence microfluidic chip for multi-marker detection, including: a top plate 1, the top plate 1 is provided with at least one sample adding part 11, a mixing area 12 interconnected with the sample adding part 11, And a plurality of labeling ligands arranged in the mixing area 12, each labeling ligand is different from each other; the bottom plate 2 arranged on the top plate 1, the bottom plate 2 includes a guide area 21 that communicates with the mixing area 12, and the guide area 21 The reaction zone 22 communicated with each other, a plurality of detection zones communicated with the reaction zone 22, the cleaning liquid storage part 24 and the luminescent liquid storage part 25 communicated with the detection zone; the reaction zone 22 is provided with a plurality of magnetic particle ligands, magnetic The particle ligands include magnetic particles and ligands, and the magnetic p...
Embodiment 2
[0034] On the basis of the first embodiment, at least one of the core-to-mass ratio, the mass and the volume of the magnetic particles in the second embodiment is different. Each magnetic particle ligand is placed in the reaction zone 22, and the external magnet moves, which can drive the magnetic particles to move. By controlling the moving speed of the magnet, when the moving speed of the magnet is fast, it drives the magnetic particles with large nuclear-to-mass ratio, high mass or large volume to move; when the moving speed of the magnet is slow, it drives the magnetic particles with small nuclear-to-mass ratio and mass. Smaller or smaller magnetic particles move. Therefore, the moving speed of the magnet can be controlled, and the magnetic particles matching the moving speed can be driven to move, even if other magnetic particles will move, but there is a channel between the reaction area 22 and the detection area, only the movement of the magnet Only magnetic particles ...
Embodiment 3
[0036] On the basis of Embodiment 1, positive electrodes and negative electrodes are respectively provided on both sides of the detection area channel in Embodiment 3, figure 1 The positions of the positive and negative electrodes are shown, as figure 1 The "+" sign shown is the position of the positive electrode, as in figure 1 The shown "-" is the position of the negative electrode. Of course, in other embodiments, the positive electrode and the negative electrode may also be located at other positions, which will not be repeated here. The isoelectric point of each magnetic particle is different, and the isoelectric point refers to the pH value when the surface of the magnetic particle is not charged. Each magnetic particle ligand is placed in the reaction zone 22, and the external magnet moves, which can drive the magnetic particles to move. After the magnet drives the magnetic particles from the reaction zone 22 to the detection zone, the magnets are withdrawn. Since the...
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