Application to preparation of gastrointestinal mucosa repair protective agent by using ANKRD22 as target

A gastrointestinal mucosal and protective agent technology, applied in the field of biomedicine, can solve problems such as increased adverse reactions, achieve the effects of increasing gastric mucus secretion, increasing gastric mucus secretion, and reducing gastric tissue inflammation

Active Publication Date: 2020-01-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these drugs have good therapeutic effects, there

Method used

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  • Application to preparation of gastrointestinal mucosa repair protective agent by using ANKRD22 as target
  • Application to preparation of gastrointestinal mucosa repair protective agent by using ANKRD22 as target
  • Application to preparation of gastrointestinal mucosa repair protective agent by using ANKRD22 as target

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1. Interfering with the gene expression of ANKRD22 can effectively alleviate the symptoms of mild to moderate gastrointestinal mucosal injury in experimental mice caused by hydrochloric acid ethanol solution (150 mM HCl / 60% absolute ethanol solution, HCl / EtOH).

[0051] In this example, it was verified that interfering with the gene expression of ANKRD22 can effectively repair the symptoms of mild to moderate gastrointestinal mucosal injury in experimental mice caused by HCl / EtOH. Among them, an experimental mouse model of mild to moderate gastrointestinal mucosal injury induced by HCl / EtOH was established. However, compared with wild-type mice, the symptoms of gastrointestinal mucosal injury in ANKRD22 knockout mice were significantly alleviated, including: fewer gastrointestinal mucosal bleeding points, and less uneven and granular mucosa.

[0052] details as follows:

[0053] HCl / EtOH Experimental Mouse Model of Mild to Moderate Gastrointestinal Mucosa Injur...

Embodiment 2

[0063] Example 2. Interfering with the gene expression of ANKRD22 can significantly increase the number of expanded LGR5+ stem cells in the gastric tissue of experimental mice with mild or moderate gastrointestinal mucosal injury.

[0064] figure 2 The effect of deletion of ANKRD22 gene on the level of LGR5 in gastric tissue of mice after 24 repair of HCl / EtOH-induced mild and moderate gastrointestinal mucosal injury is shown.

[0065] Grinding mouse gastric tissue with Trizol, extracting RNA, reverse-transcribing into cDNA, and using real-time PCR to detect the relative expression of LGR5: Ankrd22- / - mouse gastric tissue 24 hours after HCl / EtOH mild to moderate gastrointestinal mucosal injury (Ankrd22- / -HCl / EtOH) LGR5 levels were significantly increased compared with wild-type mice (Ankrd22- / -control). P<0.05 means statistically significant difference.

[0066] pass figure 1 and figure 2 The results showed that ANKRD22 gene deletion can repair mild to moderate gastroint...

Embodiment 3

[0067] Example 3. Interfering with the gene expression of ANKRD22 can significantly increase the gastric tissue mucus secretion level of experimental mice with mild or moderate gastrointestinal mucosal injury.

[0068] image 3 The effects of ANKRD22 gene deletion on the levels of Mucin2, Mucin5AC and Mucin6 in gastric tissue of mice after 24 repair of HCl / EtOH-induced mild and moderate gastrointestinal mucosal injury are shown.

[0069] After grinding mouse stomach tissue with Trizol, RNA was extracted and reverse-transcribed into cDNA, and the relative expression levels of Mucin2, Mucin5AC and Mucin6 were detected by real-time PCR: Ankrd22- / - 24 hours after HCl / EtOH mild to moderate gastrointestinal mucosal injury The levels of Mucin2, Mucin5AC, and Mucin6 in stomach tissue of mice (Ankrd22- / -HCl / EtOH) were significantly higher than those of wild-type mice (Ankrd22- / -control). P<0.05 means statistically significant difference.

[0070] image 3 It shows that the deletion ...

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Abstract

The invention discloses an application to preparation of a gastrointestinal mucosa repair protective agent by using ANKRD22 as a target. The ANKRD22 is used as the target, namely gene knock-out, geneknock-out and reduction or chemicals can reduce expression of an ANKRD22 gene. The invention provides a new target for repair of gastrointestinal mucosa light and moderate injury, namely ANKRD22 geneexpression is restrained, so that stomach tissue LGR5+ stem cells can be amplified, a stomach tissue inflammatory reaction can be alleviated, and the stomach mucosa secretion can be increased.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of targeting ANKRD22 in the preparation of a gastrointestinal mucosal repair and protection agent. Background technique [0002] The gastrointestinal tract is an important organ of digestion and absorption in the human body. Food needs to be fully ground by the stomach and mixed with gastric acid to form a paste-like chyme that can be absorbed by the small intestine. Although the gastrointestinal mucosa can protect the stomach from damage, it is extremely vulnerable to damage from diet, drugs and bad emotions. Gastrointestinal mucosal injury is a common and frequently-occurring disease worldwide, and almost all people suffer from it in their lifetime. With the increase of people's social activities, the incidence of diseases related to gastrointestinal mucosal injury caused by exogenous injury factors such as alcohol and irritating food is also increasing year by year,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N5/10
CPCC07K14/47C12Q1/6883C12Q2600/158
Inventor 朱永良柳景文
Owner ZHEJIANG UNIV
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