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Fast, simple and convenient method for preparing high-potency positive serum of riemerella anatipestifer

A technology of Reeseri and positive serum, applied in chemical instruments and methods, from serum immunoglobulin, immunoglobulin, etc., can solve the problems of long cycle, vaccine emulsification and cumbersome preparation, etc., and achieve simple operation and high bacterial content low effect

Pending Publication Date: 2020-01-10
山东和康源生物育种股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it mainly relies on preparing the oil emulsion vaccine of L. anatipestifer, immunizing SPF chickens, and preparing hyperimmune serum after multiple immunizations; the disadvantage is that the cycle is long, and the experimental animals are homologous animals, which are prone to non-specific agglutination; vaccine emulsification and Preparation is relatively cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, a kind of quick and easy preparation method of high titer positive serum of R. anatipestifer comprises the following steps:

[0033] 1) Culture medium preparation

[0034] The culture medium is divided into liquid medium and solid medium; the solid medium adopts the conventional method: Martin’s medium is added with 5% bovine serum; the base liquid of the liquid medium is Martin’s medium, and 5% of bovine serum is added before inoculation For serum and B1, add 0.1g of B1 to 10,000 ml of culture medium;

[0035] 2) Bacterial solution preparation

[0036] The pre-prepared serum strains were inoculated into solid medium; cultivated in 5% carbon dioxide medium at 37°C for 20 hours; the strains were collected, prepared into a bacterial solution of 10 billion cfu / ml, inoculated into liquid medium, and added at a ratio of 5%. The volume of the liquid medium and the volume of the container are controlled at 1:5, cultured on a shaker at 37°C, the speed is control...

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PUM

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Abstract

The invention relates to a fast, simple and convenient method for preparing a high-potency positive serum of riemerella anatipestifer, and belongs to the field of biological products. The fast, simpleand convenient method includes the following steps that (1) a culture medium is prepared, specifically, the culture medium is divided into a liquid culture medium and a solid culture medium; (2) a bacteria solution is prepared; (3) the bacteria solution is inactivated; (4) immune antibodies are prepared; (5) immunization is conducted; (6) antibody titer detection is conducted; and (7) specific detection is conducted. According to the method, rabbits are used as immune objects, inactivated antigens containing the appropriate use amount of the riemerella anatipestifer are used for immunizationto the rabbits, hyper-immune serum is produced at 7 days, and tube agglutinating antibodies can reach 1:16.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a rapid and simple preparation method for high-titer positive serum of R. anatipestifer. Background technique [0002] R. anatipestifer, also known as duck infectious serositis, is a contact, acute or chronic, septic infectious disease. The main features are fibrinous pericarditis, fibrinous perihepatitis, fibrinous air sacitis, caseous salpingitis, arthritis and paralysis. [0003] Duck infectious serositis is a chronic or acute septicemia infectious disease of ducks / geese caused by R. anatipestifer. Mainly infect ducks, geese, chickens and some wild birds can also be infected. Manifested as depression, anorexia, tearing, soft feet, thin and yellow-white or green feces, tilting the head and twisting the neck, turning in circles, intermittent convulsions, until death. Dissection shows increased pericardial fluid or adhesion with epicardium, liver enlarg...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/06
CPCC07K16/1203C07K16/06
Inventor 孟照洁翁立雪赵杰赵情
Owner 山东和康源生物育种股份有限公司
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