Manufacturing method for gene transport speed control device based on movement protein

Abstract

The invention provides a manufacturing method of a gene transport speed control device based on a movement protein. The manufacturing method comprises the following steps: i) the movement protein witha polyanion tail is formed; and ii) a nanopore device is provided, the device comprises a shell, a fluid cavity sealing layer, a flexible substrate nanopore structure and a fluid cavity base; the flexible substrate nanopore structure comprises a metal layer to reduce the nanopore size to 1-100 nm; the movement protein is stabilized in nanopore; and the movement protein is anchored into the nanopore through a metal-sulfur bond, so that the transport speed of a gene chain to be detected passing through the nanopore is at least slower than 0.1 ms per base pair. According to the method, the movement protein is anchored in the nanopore by using a click chemistry process, so that the transport speed of the gene chain to be detected passing through the nanopore is at least slower than 0.1 ms perbase pair; and the temperature and the pH value are adjusted, so that the transport speed of the gene chain can be effectively controlled, the transport speed of the gene chain to be detected passingthrough the nanopore 51 can be further reduced to tens of milliseconds or even hundreds of milliseconds, and the sensitivity of spectrum detection can be improved.

Description

technical field [0001] The invention belongs to the fields of gene sequencing and biomolecular sensing, and in particular relates to a method for manufacturing a motor protein-based gene transfer speed control device. Background technique [0002] The measurement of genetic information has revolutionized the field of life sciences and medicine. Future precision medicine and personalized medicine require new sequencing technologies with lower cost, faster speed, higher precision and longer read length. [0003] The new generation of single-molecule real-time sequencing technology addresses the need for longer read length and faster speed; the recent rapid development of biological nanopore sequencing technology further addresses the need for lower cost. Biological nanopore sequencing technology does not need to prepare a large number of samples, and the sample preparation process does not need to consume biological and chemical reagents, which greatly reduces the cost of seq...

AI Technical Summary

Problems solved by technology

The current solid-state nanopore technology mainly realizes sequencing by measuring the ion blocking current, but it faces many challenges: first, the translocation behavior of the DNA chain in the nanopore is not easy to control, and the direction of the base is uncontrolled, which has great Randomness, DNA moves too fast (0.1-1μs / bp); DNA binds non-specifically to the surface of the nanopore to form a secondary or tertiary structure, blocks the nanopore, and restricts the normal translocation of the DNA chain; in traditional biological In solid-state nanopore detection technology, ion blocking current is generally used to distinguish different base sequences. However, current detection technology has an essential limitation: the electric field around the nanopore will extend to both sides, resulting in the extension of the effective length of the nanopore. , which limits the detection resolution

These problems have severely limited the successful implementation of solid-state nanopore sequencing technology.

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