Gene transport speed control device based on kinetin

Abstract

The invention provides a gene transport speed control device based on kinetin. The device comprises kinetin, a shell, a fluid cavity sealing layer, a flexible substrate nanopore structure, a fluid cavity base, a power supply, a first electrode and a second electrode, the nanopore structure comprises nanopores and a metal layer, and the size of the nanopores can be reduced to 1-100nm; and the kinetin and the metal layer form a metal-sulfur bond, so that the transport speed of the gene chain to be detected passing through the nanopores is at least lower than 0.1 ms / base pair. According to the invention, the kinetin is anchored in the nanopores by using a click chemistry method, so that the transport speed of the gene chain to be detected passing through the nanopores is at least slower than0.1 ms / base pair; and then the temperature and the pH value are adjusted to effectively control the transport speed of the gene chain, the transport speed of the gene chain to be detected passing through the nanopores 51 can be further reduced to tens-of-millisecond level or even hundreds-of-millisecond level, and the sensitivity of spectrum detection is improved.

Description

technical field [0001] The invention belongs to the field of gene sequencing and biomolecular sensing, and in particular relates to a motor protein-based gene transport speed control device. Background technique [0002] The measurement of genetic information has revolutionized the fields of life sciences and medicine. The future of precision medicine and personalized medicine requires new sequencing technologies with lower cost, faster speed, higher precision and longer read length. [0003] Next-generation single-molecule real-time sequencing technology addresses the need for longer read lengths and faster speeds; the recent rapid development of biological nanopore sequencing technologies further addresses the need for lower costs. The biological nanopore sequencing technology does not require the preparation of a large number of samples, and the sample preparation process does not require the consumption of biological and chemical reagents, which greatly reduces the cost...

AI Technical Summary

Problems solved by technology

The current solid-state nanopore technology mainly realizes sequencing by measuring the ion blocking current, but it faces many challenges: first, the translocation behavior of the DNA chain in the nanopore is not easy to control, and the direction of the base is uncontrolled, which has great Randomness, DNA moves too fast (0.1-1μs / bp); DNA binds non-specifically to the surface of the nanopore to form a secondary or tertiary structure, blocks the nanopore, and restricts the normal translocation of the DNA chain; in traditional biological In solid-state nanopore detection technology, ion blocking current is generally used to distinguish different base sequences. However, current detection technology has an essential limitation: the electric field around the nanopore will extend to both sides, resulting in the extension of the effective length of the nanopore. , which limits the detection resolution

These problems have severely limited the successful implementation of solid-state nanopore sequencing technology.

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