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SAA gene related to tridacna immunity and application of gene in preparing tridacna pathological detection reagent

A technology related to immunity and detection reagents, applied in the field of molecular biology, can solve the problems of few research reports and the trough of giant clam research, so as to improve the ability of germplasm innovation and prevent the expansion of disasters

Active Publication Date: 2020-01-10
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

But then, people's research on giant clams entered a trough, and so far there are few related research reports

Method used

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  • SAA gene related to tridacna immunity and application of gene in preparing tridacna pathological detection reagent
  • SAA gene related to tridacna immunity and application of gene in preparing tridacna pathological detection reagent
  • SAA gene related to tridacna immunity and application of gene in preparing tridacna pathological detection reagent

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with embodiment. The examples are intended to illustrate the present invention, but not to limit the present invention in any form.

[0026] 1. Provide tissue samples of Tridacna safranata in accordance with the sample submission requirements of Nuohezhiyuan Bioinformation Technology Co., Ltd. without reference. Select 5 individuals with an average shell height of 7±1 cm from safflower clam, take 0.1 g each of the mantle, obturator muscle, gonad, and lymphocytes or tissues, add Trizol (Invitrogen, USA), and grind with a tissue grinder , put it into liquid nitrogen and freeze immediately, and deliver it to the company by dry ice express the next day, and carry out the next step of experimental processing according to each tissue sample, including RNA extraction, quality inspection, transcription, library construction, and Illumina HiSeq sequencing. The simple process is as follows figure 1 .

[0027] ...

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Abstract

The invention discloses an SAA gene related to tridacna immunity and an application of the gene in preparing a tridacna pathological detection reagent. In the invention, the SAA gene is cloned from tridacna crocea for the first time, and a universal primer TcSAA-F / TcSAA-R is designed for the gene in several common tridacna. Quantitative PCR experiments show that the SAA gene of the tridacna croceais significantly up-regulated after vibrio infection and very similar to that of mammals, and in other pathological conditions, the expression of the gene is also significantly up-regulated. Therefore, the SAA gene can also be used as a gold standard for detecting tridacna health conditions. The SAA gene and the primer TcSAA-F / TcSAA-R provided by the invention can be used to monitor the health conditions of the tridacna, provide a positive and accurate warning signal for the prevention of tridacna diseases, and can effectively prevent the expansion of disasters; and the application of the technology further improves the ability of tridacna breeding germplasm innovation and supports the efficient and sustainable development of industry.

Description

Technical field: [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SAA gene related to immunity of giant clams and its application in preparing reagents for detecting the pathology of giant clams. Background technique: [0002] Tridacna belongs to the phylum Molluscs, Phyllobranchia, Pleurobranches, and Tridacnaidae. There are 2 genera. There are 9 species in the genus Tridacna, and only 1 species in the genus Tridacna. Tridacna is the largest shellfish today, and some species can reach about 1 meter in size and weigh more than 300 kilograms. In addition to being the largest in size, Tridacna can also be self-supporting, because there are symbiotic zooxanthellae in its mantle , can carry out photosynthesis under light conditions, provide nutrients for giant clams, enable them to survive in the open sea where nutrients are extremely deficient, and provide the cornerstone for the construction of coral reefs. Tridacna are ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/11C07K14/775C07K14/435C12Q1/6883
CPCC07K14/43504C07K14/775C12Q1/6883
Inventor 向志明赵泽慧张跃环肖述张扬李军喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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