Unlock instant, AI-driven research and patent intelligence for your innovation.
Anti-tumor polypeptide sur-x targeting survivin-xiap complex and its use
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of sur-x and compound, applied in the field of -XIAP compound, can solve the problems of increased expression of XIAP and increased risk of tumor recurrence
Active Publication Date: 2021-04-20
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
View PDF4 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
In patients with basal-like breast cancer, XIAP expression is elevated and the risk of tumor recurrence is significantly increased
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0033]Example 1 Synthesis, Purification and Molecular Measurement of Polypeptide S
[0034]The anti-tumor polypeptide S in the present invention is a particular amino acid sequence that binds to XIAP according to the uneven document, and is incorporated herein by reference. The final sequence is in SEQ ID NO: 1. Select a peptide segment that does not expressly expresses in human cells as a control of the polypeptide S having a specific anti-tumor of the present invention, which is recorded as a polypeptide CON, and its final sequence is seen in SEQ ID NO: 2. The polypeptide NS is a shortened polypeptide S to obtain a residue of two-thirds of the S amino group. The final sequence of the NS is shown in SEQ ID NO: 3. Both polypeptides S, CON and NS were synthesized by FMOC solidpeptide synthesis, as follows: According to the polypeptide sequence, the peptide chain is delayed from the carboxy terminal to the amino terminal, and after the peptide is synthesized, the 95 cutting fluid (three...
Embodiment 2
[0037]Example 2 Observation of the polypeptide under the copolymerization microscope can enter the cell
[0038]HoecHst33342 was treated with a polypeptide into a cell under a copolymerized microscope at a common focusing microscope at a copolymerized microscope to treat human colorectal cancer cells HCT 116 and RKO 1H at a ratio of 1: 1000.
[0039]image 3 The polypeptide S and CON can be observed to be observed under a copolymerized microscope, and the polypeptide S has a good film passivity.
Embodiment 3
[0040]Example 3MTT Detection S on the human nuts rectal cancer cell line HCT116 and RKO, human gastric cancer cell line MGC-803 and MKN45, and human normal cell line SV5 cell viability
[0041]The logarithmic cells, counting cells were collected, and the cell concentration was adjusted, and in 5,000 cells / well were seeded in a 96-well culture plate; set the experimental group (S) and a negative control group (CON), each set of concentration gradients: 0, 2.5 μm, 5 μm, 10 μm, 15 μm, 20 μm, each concentration of 3 complexificial holes; after the cell is attached, the corresponding treatment is given, for 6 h, 24 h, respectively; 20 μl of MTT (5 g / L), incubation at 37 ° C After 4 hours, the supernatant was given, and 200 μl of dimethyl sulfoxide (DMSO) was added, and the absorbance (OD) value of the 570 nm wavelength was measured; the cells were relatively viable under different concentrations; repeated three times.
[0042]Figure 4 It is shown as the effect of HCT116 and RKO, human gast...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The present invention relates to the field of targeted therapeutic drugs for tumors, in particular to an anti-tumor polypeptide Sur-X based on targeting survivin-XIAP complex, and its application in the treatment of various tumors including colorectal cancer and gastric cancer . The anti-tumor polypeptide Sur-X of the present invention can rapidly and efficiently induce the death of colorectal cancer and gastric cancer cells by inhibiting the formation of the survivin-XIAP complex, and has no effect on normal human cells, thereby exerting the therapeutic effect of specifically targeting tumor cells. In addition, the polypeptide is easy to synthesize in vitro, which is convenient for clinical application. Therefore, the polypeptide Sur‑X has potential commercial value and can be widely used in the field of tumor targeting therapy technology.
Description
Technical field[0001]The present invention relates to a tumor targeting therapeutic pharmaceutical sector, and more particularly to a polypeptide drug SUR-X designed based on a survivin-xiap complex, and their applications in a variety of tumors including colorectal cancer and gastric cancer. It is particularly related to SUR-X by interfering with the formation of Survivin-XIAP complexs, promoting apoptosis and procedural necrosis of tumor cells, and exerts the role of tumor targeting treatment.Background technique[0002]In recent decades, with the extension of human average life, the change in living environment and lifestyle, cancer has become the first killer that threatens human life. Global Cancer Statistics (Globocan) showed that there were 18.1 million cancer new cases and 9.6 million cancer deaths in 2018. Among them, the incidence of colorectal cancer and gastric cancer ranked third and fifth, and the sum of two is 15.9%. In addition, the mortality rate of colorectal cancer ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More