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Method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud

A technology of Haematococcus pluvialis and astaxanthin, applied in the directions of separation method, solvent extraction, solid solvent extraction, etc., can solve the problems of destroying the effective components of astaxanthin, reducing the yield of astaxanthin, and poor extraction effect. , to achieve the effect of low production cost, mild conditions and reduced blockage

Active Publication Date: 2021-06-01
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional astaxanthin extraction methods: solvent extraction method, supercritical extraction method and direct extraction method, etc., solution extraction method and supercritical extraction method generally require the moisture content to be lower than about 5%, and hot air drying is often used to remove moisture , but high temperature and contact with oxygen will cause the loss of active components of astaxanthin, the loss rate is up to about 40%, reducing the yield of astaxanthin; direct extraction method is to extract astaxanthin directly from wet algae mud, currently There are few research reports on the process of extracting astaxanthin directly from wet algae mud, and the extraction effect is not good. Ren Xiaoli et al. used wet algae mud to extract astaxanthin by breaking the wall of the organic solvent with an acid method. This method eliminates the drying process. However, the acid method will also destroy the effective components of astaxanthin, and its extraction rate is only 69.35%. Therefore, it is urgent to provide a method for extracting astaxanthin with a high extraction rate.

Method used

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  • Method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud
  • Method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud
  • Method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud

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Embodiment 1

[0036] Such as figure 1 As shown, the method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud comprises the following steps:

[0037] Step 1. Collect the Haematococcus pluvialis after the cultivation (astaxanthin accounts for 3.09% of the dry weight of the algae cells) and wash and centrifuge to obtain the Haematococcus pluvialis algae mud (moisture content is 72%). The algae algae mud is placed in a low-temperature mechanical crushing (high-speed impact shearing extrusion type) equipment for mechanical wall breaking, the frequency is 50 Hz, the mechanical wall breaking time is 10 minutes, and the temperature is -10 ° C to obtain algal pulp;

[0038] Step 2, adding ethanol (using a mass fraction of 95%) to the algal pulp, mixing evenly and centrifuging to obtain a precipitate, wherein the mass ratio of the algal pulp to ethanol is 1:5;

[0039] Step 3. Add ethanol (mass fraction: 95%) to the precipitate and heat and stir at 50°C for 20 minutes for...

Embodiment 2

[0042] Such as figure 1 As shown, the method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud comprises the following steps:

[0043]Step 1. Collect the Haematococcus pluvialis that has been cultured (astaxanthin accounts for 3.09% of the dry weight of the algae cells) and wash and centrifuge to obtain the Haematococcus pluvialls algae mud (moisture content is 72%). The algae algae mud is placed in a low-temperature mechanical crushing (high-speed impact shearing extrusion type) equipment for mechanical wall breaking, the frequency is 70 Hz, the mechanical wall breaking time is 20 minutes, and the temperature is -10 ° C to obtain algal pulp;

[0044] Step 2, adding ethanol (using a mass fraction of 95%) to the algal pulp, mixing evenly and centrifuging to obtain a precipitate, wherein the mass ratio of the algal pulp to ethanol is 1:5;

[0045] Step 3. Add ethanol (mass fraction: 95%) to the precipitate and heat and stir at 60°C for 40 minutes for...

Embodiment 3

[0048] Such as figure 1 As shown, the method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud comprises the following steps:

[0049] Step 1. Collect the Haematococcus pluvialis that has been cultured (astaxanthin accounts for 3.09% of the dry weight of the algae cells) and wash and centrifuge to obtain the Haematococcus pluvialls algae mud (moisture content is 72%). The algae algae mud is placed in a low-temperature mechanical crushing (high-speed impact shear extrusion) equipment for mechanical wall breaking, the frequency is 60 Hz, the mechanical wall breaking time is 10 minutes, and the temperature is -20 ° C to obtain algal pulp;

[0050] Step 2, adding ethanol (using a mass fraction of 95%) to the algal pulp, mixing evenly and centrifuging to obtain a precipitate, wherein the mass ratio of the algal pulp to ethanol is 1:5;

[0051] Step 3. Add ethanol (mass fraction: 95%) to the precipitate and heat and stir at 40°C for 10 minutes for leachi...

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Abstract

The invention discloses a method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud, comprising the following steps: step 1, placing the Haematococcus pluvialis algae mud in a low-temperature mechanical crushing device for mechanical wall breaking, Obtain algal pulp; step 2, add ethanol to the algal pulp and centrifuge to obtain a precipitate; step 3, add an organic solvent to the precipitate and heat and stir for leaching and extraction, and repeatedly leaching and extracting for many times until the extract is Colorless, the extracts were combined and concentrated to obtain astaxanthin. In step 1, the preparation method of Haematococcus pluvialis algae mud is as follows: the Haematococcus pluvialis algae mud that has been collected and cultivated is sequentially washed with water and centrifuged to obtain the Haematococcus pluvialls algae mud. The invention adopts the combination method of low-temperature mechanical wall breaking and organic solvent extraction to extract astaxanthin, which can avoid the damage of high temperature to the active components of Haematococcus pluvialis, and improve the extraction rate of astaxanthin.

Description

technical field [0001] The invention relates to the field of biochemical technology. More specifically, the present invention relates to a method for efficiently extracting astaxanthin from Haematococcus pluvialis algae mud. Background technique [0002] Astaxanthin (astaxanthin), a red pigment, is the strongest natural antioxidant known in nature. Haematococcus pluvialis is the best biological source of natural astaxanthin in nature, and its accumulated astaxanthin is as high as 1.0% to 6.0%, which is a concentrated product of natural astaxanthin, and astaxanthin in Most of the primes are 3S-3'S type. Therefore, the use of this microalgae to extract astaxanthin has a very broad development prospect. Conventional astaxanthin extraction methods: solvent extraction method, supercritical extraction method and direct extraction method, etc., solution extraction method and supercritical extraction method generally require the moisture content to be lower than about 5%, and hot ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24B01D11/02
CPCB01D11/0257B01D11/0288C07C403/24C07C2601/16
Inventor 李倩郑玉彬代云法谢诗懿杨义高第丰曾继蛟
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING