Application of HBP protein as diagnostic marker of Kawasaki disease

A technology for diagnosing markers and Kawasaki disease, applied in the field of plasma protein markers, to achieve the effects of good sensitivity, high accuracy and high sensitivity

Active Publication Date: 2020-01-24
NANJING CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of HBP as a molecular marker in the diagnosis of Kawasaki disease

Method used

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  • Application of HBP protein as diagnostic marker of Kawasaki disease
  • Application of HBP protein as diagnostic marker of Kawasaki disease
  • Application of HBP protein as diagnostic marker of Kawasaki disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Patients with Kawasaki disease have higher plasma HBP levels in the acute phase

[0054] The detection results of HBP expression level in the plasma samples of healthy control group, fever group and Kawasaki disease group: the relative expression level of HBP in the control group was 7.6±2.7ng / mL, that of the fever group was 33.8±24.8ng / mL, and that of the Kawasaki disease group was 7.6±2.7ng / mL. Group was 77.1 ± 27.7 ng / mL. Statistical analysis was carried out on the relative expression levels of HBP in the samples of each group, and it was found that there were significant differences in plasma HBP between the normal group VS Kawasaki disease group, the fever group VS Kawasaki disease group (pfigure 1 ).

[0055] Taking the fever group as the control group and the Kawasaki disease group as the disease group, ROC curve analysis was performed on the relative expression levels of HBP, ESR, and CRP, and the AUC values ​​were HBP=0.88, ESR=0.73, and CRP=0.73. T...

Embodiment 2

[0057] Example 2: The plasma HBP level of patients with Kawasaki disease is significantly elevated in patients with gamma globulin non-responsive Kawasaki disease.

[0058] The detection results of HBP expression level in the plasma samples of the gamma globulin reaction Kawasaki disease group and non-reaction group: the relative expression level of HBP in the gamma globulin reaction Kawasaki disease group was 74.3±26.2 ng / mL as the mean ± standard deviation, and 74.3 ± 26.2 ng / mL in the non-reactive group. The response group was 94.7±30.6 ng / mL. Plasma HBP was significantly different between gamma globulin reactive Kawasaki disease group and non-responsive group (p image 3 ), its Cutoff value is 98.5ng / mL.

Embodiment 3

[0059] Example 3: The level of plasma HBP in patients with Kawasaki disease is significantly increased in patients with coronary artery injury in Kawasaki disease.

[0060] The detection results of HBP expression level in the plasma samples of the Kawasaki disease group without coronary artery injury and the Kawasaki disease group with coronary artery injury: the mean ± standard deviation of HBP in the Kawasaki disease group without coronary artery injury was 72.5±25.3, and that in the Kawasaki disease group with arterial injury was 72.5±25.3 96.4±29.3. Plasma HBP was significantly different between the Kawasaki disease group without coronary artery injury and the coronary artery injury group (p Figure 4 ), its Cutoff value is 83ng / mL.

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Abstract

The invention provides application of HBP protein as a diagnostic marker of Kawasaki disease, and discloses application of HBP protein as a diagnostic marker of Kawasaki disease in preparing diagnostic products of Kawasaki disease and a kit for diagnosing the Kawasaki disease. The experimental results show that the plasma HBP level in acute Kawasaki patients is significantly higher than that in healthy children and general patients infected with fever, the higher HBP concentration is significantly correlated with coronary artery injury in Kawasaki disease, and the HBP level is higher in insensitive patients treated with IVIG than in sensitive patients treated with IVIG. The invention finds that plasma HBP can be used to differentiate Kawasaki disease from infected fever patients, wherein the area under ROC curve (area under the curve, AUC) is 0.88, the optimal diagnostic threshold is 52.5pg/mL, the sensitivity is 88.3%, and the specificity is 74.0%. Therefore, plasma HBP protein of patients can be used as a potential marker for distinguishing diagnosis of Kawasaki disease from infected fever.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plasma protein marker related to Kawasaki disease and its application. Background technique [0002] Kawasaki disease (KD) is an immune-mediated acute systemic vasculitis of small and medium-sized vessels. Kawasaki disease is highly prevalent in Asia, mostly in infants under 5 years old. The pericardium, myocardium, endocardium and coronary artery can be involved in the early stage, and the incidence of cardiovascular injury caused by it is about 25.4%. The most serious is coronary artery injury (CAL), which accounts for about 68% of cardiovascular injury, including Coronary artery dilation, stenosis, coronary aneurysm formation, and even myocardial infarction in severe cases. In recent years, KD has been increasing year by year, and has become the main cause of acquired heart disease in children, and it is also an important cause of coronary artery disease in young and middle-age...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/328G01N2333/47
Inventor 龙广凤陈红兵徐诚夏微微杨大恒
Owner NANJING CHILDRENS HOSPITAL
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