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Microbe and plant combined in-situ remediation process for petroleum-contaminated soil

A soil microorganism and oil pollution technology, which is applied in the field of oil-contaminated soil microorganism and plant joint in-situ remediation process, can solve the problems such as the difficulty of microorganisms ingesting pollutants and the migration of exogenous microorganisms, so as to improve the repair effect and adsorption effect , the effect of improving fertility

Inactive Publication Date: 2020-02-04
东营金岛环境工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most abiotic factors can be regulated by engineering measures, such as adjusting soil water content, pH, adding organic carbon sources and inorganic nutrients, etc.; Difficulty in the migration of microorganisms, resulting in the inability of microorganisms to effectively contact and ingest pollutants. Difficulty in the uptake of pollutants by microorganisms is a bottleneck in in-situ bioremediation

Method used

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  • Microbe and plant combined in-situ remediation process for petroleum-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, characterized in that it mainly includes the following steps:

[0030] (1) Get the soil polluted by oil and carry out flora screening;

[0031] (2) mixing the microorganisms obtained in step (1) with sodium alginate solution, and adding chitosan solution, crosslinking agent and cotton seeds, after stirring and reacting, the modified cotton seeds are obtained;

[0032] (3) Add the fertilizer slow-release agent to the soil contaminated by oil, and control the soil water content, and let it stand for reaction;

[0033] (4) Applying the modified cotton seeds obtained in the step (2) to the oil-contaminated soil treated in the step (3), controlling the water content of the soil, and culturing for 6 months.

[0034] As an optimization, the combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, the main processing steps are as follows:

[0035] (...

Embodiment 2

[0046] A combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, characterized in that it mainly includes the following steps:

[0047] (1) Get the soil polluted by oil and carry out flora screening;

[0048] (2) mixing the microorganisms obtained in step (1) with sodium alginate solution, and adding chitosan solution, crosslinking agent and cotton seeds, after stirring and reacting, the modified cotton seeds are obtained;

[0049] (3) Applying the modified cotton seeds obtained in the step (2) to the soil polluted by oil, controlling the water content of the soil, and culturing for 6 months.

[0050] As an optimization, the combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, the main processing steps are as follows:

[0051] (1) Take oil-contaminated soil for flora screening:

[0052] (a) Preliminary screening: put 5g of oil-contaminated soil into 200mL bacterial culture solution, and culture at a c...

Embodiment 3

[0060] A combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, characterized in that it mainly includes the following steps:

[0061] (1) Get the soil polluted by oil and carry out flora screening;

[0062] (2) mixing the microorganisms obtained in step (1) with sodium alginate solution, and adding chitosan solution, cross-linking agent and humic acid, after stirring and reacting, modified humic acid is obtained;

[0063] (3) Add the fertilizer slow-release agent to the soil contaminated by oil, and control the soil water content, and let it stand for reaction;

[0064] (4) Applying the modified humic acid obtained in the step (2) to the petroleum-contaminated soil treated in the step (3), controlling the soil water content, and culturing for 6 months.

[0065] As an optimization, the combined in-situ remediation process of microorganisms and plants in oil-contaminated soil, the main processing steps are as follows:

[0066] (1) Take oil...

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Abstract

The invention discloses a microbe and plant combined in-situ remediation process for petroleum-contaminated soil and relates to the technical field of soil remediation. The microbe and plant combinedin-situ remediation process for the petroleum-contaminated soil, disclosed by the invention, comprises the main remediation steps: (1) screening petroleum degrading bacteria; (2) preparing modified cotton seeds; (3) applying a fertilizer sustained-release agent; and (4) applying the modified cotton seeds. According to the remediation process disclosed by the invention, by adding the homemade wastesustained-release agent and the modified cotton seeds, the soil remediation effect of the remediation process can be effectively improved, and the content of petroleum in the petroleum-contaminated soil is greatly lowered.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a combined in-situ remediation process of petroleum-contaminated soil microorganisms and plants. Background technique [0002] The bioremediation technology of oil-contaminated soil can be divided into natural attenuation method, biostimulation method and bioaugmentation method according to the specific operation and the source of bacteria. Natural decay and biostimulation have low efficiency in degrading pollutants and tend to produce intermediate metabolites with stronger mobility, so they are less and less used. The method of screening microorganisms that can efficiently degrade petroleum hydrocarbon pollutants through microbial operations, and adding them to contaminated soil as exogenous high-efficiency bacteria to degrade and transform petroleum hydrocarbon pollutants is the bioaugmentation method. [0003] Theoretically, bioaugmented remediation technology can ac...

Claims

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Application Information

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IPC IPC(8): B09C1/10C05G3/80C05G3/90C05G3/40
CPCB09C1/10B09C1/105B09C2101/00C05B1/02C05G3/00
Inventor 于国明张俊锋张晓飞刘东刘德华
Owner 东营金岛环境工程有限公司