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Seedling strengthening and controlling method in rice anther cultivation process

A technology for rice and anthers, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of unsuitable planting of cultured seedlings, senescence and weakening of cultured seedlings, etc., so as to improve callus differentiation rate and prolong growth. time, the effect of improving growth potential

Pending Publication Date: 2020-02-07
COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limited temperature conditions in the north, the cultured seedlings are not suitable for planting in the natural environment
While the cultured seedlings continue to grow in the rice medium, the cultured seedlings are prone to premature aging and weakening

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present invention is based on the theory of rice anther culture, and according to the principle that paclobutrazol can regulate rice cell differentiation in the process of rice anther culture, it is screened out that the concentration of paclobutrazol added to the differentiation medium is 2mg / L, which can prolong the growth time of differentiated seedlings by 50-60 days By 70 to 90 days, the number of tillers of differentiated seedlings is increased from 2 to 3 to 5 to 6 under optimal conditions, the differentiation rate of green seedlings reaches more than 80%, and the rate of differentiation of green seedlings is increased by more than 10%. A method of adding paclobutrazol at a concentration of 5 mg / L to the rooting medium is screened out to make the cultured seedlings grow normally for 100-120 days, and the root system of the cultured seedlings increases by 20% compared with the conventional rice anther culture method.

[0023] The specific culture medium system ...

Embodiment 2

[0027] 1) Take seeds

[0028] Seeding began in late July. Choose plump rice with no exposed ears and when the length of the tip leaf and the second leaf stalk of the rice reaches 3-5cm, cut it off along the root, at least 5 plants for each variety, mark the name of the variety and hang a sign.

[0029] 2) Handling and storage

[0030] Peel off the excess stems and leaves, leaving only the pointed leaves and the second leaf. Align the stalks at the root of the five baht rice and cut off the excess stems, leaving 1cm, and place them on a table sterilized with 75% ethanol. The good rice and signs are sterilized with 75% ethanol (note: the stems and leaves must be wiped), and then wrapped with gauze soaked in ultrapure water (keep some water in the gauze and do not wring it out). The processed rice is packed in a clean plastic bag and stored in a fresh-keeping refrigerator. Experiments can be performed after one week. This operation process is performed after disinfection with...

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Abstract

The invention discloses a seedling strengthening and controlling method in a rice anther cultivation process. In the rice anther cultivation process, differentiation time of japonica rice anther calluses is prolonged by the aid of special differential media and rooting media, rooting time of japonica rice anther meristems is prolonged, and the growth vigor of japonica rice anther cultivation seedlings is improved. The differential media comprise (MS), (1.5mg / L) NAA, (2mg / L) 6-BA, (2mg / L) paclobutrazol, (30g / L) sucrose and (6g / L) agar powder, and the rooting media comprise (MS), (2mg / L) NAA, (5mg / L) paclobutrazol, (30g / L) sucrose and (6g / L) agar powder. According to the method, production time of the rice anther cultivation seedlings can be prolonged, and the growth vigor of the cultivationseedlings is improved.

Description

technical field [0001] The invention belongs to the technical field of job breeding, and in particular relates to a method for strengthening seedlings and controlling seedlings in the process of cultivating rice anthers. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population depends on rice as a staple food. The advancement of breeding technology is the main driving force for the improvement of rice yield, quality, resistance and other traits. Since the invention of rice anther culture technology in the 1970s, rice anther culture technology has become an important means to improve the efficiency of rice breeding. However, it is difficult to control the time of callus differentiation and rooting of cultured seedlings during rice anther culture. Under normal conditions, rice anthers can be cultivated for 3 to 4 months to produce cultured seedlings, that is, in the north, rice heads are produced in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王永新耿雷跃张薇邹拓杜琪
Owner COASTAL AGRI RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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