Method for preparing cyclinasinensis oligopeptide

A technology of green clams and oligopeptides, applied in the field of preparing short peptides, can solve problems such as unfavorable operation and viscosity increase

Active Publication Date: 2020-02-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the good solvent properties of ionic liquids, ionic liquids are also used for peptide extraction research, but the viscosity of ionic liquids increases at low temperatures, which is very unfavorable for operation.
However, there is no research on the application of this binary system to peptide extraction.

Method used

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  • Method for preparing cyclinasinensis oligopeptide

Examples

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Embodiment 1

[0032] A preparation method of clam oligopeptide, comprising the following steps:

[0033] (1) After fresh green clams are washed, shelled, and viscera are taken, after cleaning, homogenate is processed to obtain green clam homogenate;

[0034] (2) Mix the clam homogenate and compound solvent uniformly at a material-to-liquid ratio of 1:3 to obtain a soaking solution, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0035] (3) Reduce the temperature of the soaking solution to -60°C, and pass pulse electric field treatment into the soaking solution. The number of pulses is 250, the electric field strength is 18kV / cm, the pulse width is 55μs, and the pulse frequency is 2000Hz. The soaking solution is kept at a low temperature of -60°C. Keep it for 8 hours, then quickly rise to room temperature 23±2°C within 13 minutes;

[0036] (4) The soaking solution treated in step (3) was centrifuged at 4°C for 3, and ...

Embodiment 2

[0041] A preparation method of clam oligopeptide, comprising the following steps:

[0042] (1) After fresh green clams are washed, shelled, and viscera are taken, after cleaning, homogenate is processed to obtain green clam homogenate;

[0043] (2) Mix the clam homogenate and compound solvent uniformly at a material-to-liquid ratio of 1:2 to obtain a soaking solution, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0044] (3) Reduce the temperature of the soaking solution to -55°C, pass pulse electric field treatment into the soaking solution, the number of pulses is 280, the electric field strength is 17kV / cm, the pulse width is 60μs, the pulse frequency is 1800Hz, and the soaking solution is at a low temperature of -55°C Keep for 7 hours, then rise to room temperature 23±2°C for 10 minutes;

[0045] (4) The soaking liquid treated in step (3) was centrifuged at 0°C 4, recovered the supernatant liquid s...

Embodiment 3

[0050] A preparation method of clam oligopeptide, comprising the following steps:

[0051] (1) After fresh green clams are washed, shelled, and viscera are taken, after cleaning, homogenate is processed to obtain green clam homogenate;

[0052] (2) Mix the clam homogenate and compound solvent uniformly at a material-to-liquid ratio of 1:4 to obtain a soaking solution, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0053] (3) Reduce the temperature of the soaking solution to -50°C, and pass pulse electric field treatment into the soaking solution. The number of pulses is 200, the electric field strength is 20kV / cm, the pulse width is 50μs, and the pulse frequency is 1500Hz. The soaking solution is kept at a low temperature of -50°C Keep for 7 hours, then quickly rise to room temperature 23±2°C for 20 minutes;

[0054] (4) The soaking solution treated in step (3) was centrifuged at 0°C for 5 times, and t...

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Abstract

The invention relates to the technical field of short peptides prepared from cyclinasinensis and provides a method for preparing a cyclinasinensis oligopeptide for further improving the extraction efficiency of the cyclinasinensis oligopeptide. The method comprises the following steps: applying a binary system combined by ionic liquid EMIMBF4 and gamma-butyrolactone in a volume ratio of 1:1 to preparation of the cyclinasinensis oligopeptide, introducing a pulse electric field at low temperature of negative 50-negative 60 DEG C, and performing enzymolysis treatment under an enzymolysis condition of early optimized papain. The extraction rate of the cyclinasinensis oligopeptide is improved, and the bioactivity is improved to a certain extent.

Description

technical field [0001] The invention relates to the technical field of preparing short peptides from green clams, in particular to a preparation method of clam oligopeptides. Background technique [0002] Green clams (Cyclina sinensis) belong to the mollusk phylum, branchial valve class, heterodentate subclass, clam order, clam family, contain high protein, low fat and high content of unsaturated fatty acids, delicious taste, good nutrition value. Green clam has a long history of being used as folk medicine, and is an important marine medicine, which has the functions of softening hard masses, clearing away heat and dampness, and antitussive. In the previous research, the inventor team of the present application prepared clam oligopeptides with anti-tumor effects using clams as raw materials, see Chinese patent application CN201610326511.1, the invention title is "A use of clam enzymatically hydrolyzed oligopeptides", The amino acid sequence of the clam oligopeptide obtain...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
CPCC07K7/06C12P21/06
Inventor 杨最素余方苗唐云平黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
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