A primer set, kit and detection method for rapid detection of human dys385 gene

Abstract

The invention discloses a primer set for rapidly detecting human DYS385 gene. Wherein, the primer set is composed of the following primers: primer F1 as shown in SEQ ID No.1; primer B1 as shown in SEQ ID No.2; primer F2-1 as shown in SEQ ID No.3; primer B2-1 As shown in SEQ ID No.4; Primer F2-2 as shown in SEQ ID No.5; Primer B2-2 as shown in SEQ ID No.6; Primer LOOP-F as shown in SEQ ID No.7; Primer LOOP -B is shown in SEQ ID No.8. The detection time required by the present invention is short, and the corresponding primers and kit can quickly detect the human DYS385 gene within 30 minutes to determine the sex of the human sample.

Description

technical field [0001] The invention relates to a detection kit and a method, in particular to a primer set, a kit and a detection method for rapid detection of human DYS385 gene, belonging to the field of forensic detection. Background technique [0002] Humans have a total of 46 chromosomes, of which 22 are autosomes and the other two are sex chromosomes. Females have XX and males have XY. The DNA level identification of the human Y chromosome can be achieved by amplifying the unique genes of the human Y chromosome. The X chromosome cannot amplify the target fragment, but the Y chromosome can amplify the target fragment. Relying on this principle, the currently commonly used forensic DNA gender identification method is to use an identification kit to amplify the test material in a professional laboratory environment, and to detect the amplified product on a capillary electrophoresis apparatus. By amplifying the DYS38 locus, the female sample has no amplification peak, but...

AI Technical Summary

Problems solved by technology

However, in the prior art, the amplification process after the DNA extraction of the samples takes at least 2 to 4 hours, and the detection of the amplification products usually takes about 1 hour.

In addition, it is necessary to optimize the renaturation temperature to improve the specificity during the operation, the work is cumbersome and complicated, and the reaction takes a long time

And because the amplification procedure requires a professional laboratory environment and precision instruments, it is difficult to carry out detection on site, and all of them need to be sent for inspection. The inspection process will also cause time consumption and increase the detection cost.

In the process of submitting for inspection, it is also easy to cause damage and loss of samples submitted for inspection, resulting in the loss of important evidence

In case of corrupt samples, special packaging is required. If not handled properly, it is easy to contaminate the inspectors during the inspection process.

[0005] (2) Strong professionalism: the entire detection process needs to be carried out in a professional laboratory environment, and the analysis of the experimental operation and amplification results needs to be judged by professionally trained professionals

Due to the high requirements for testing, the limited number of testing institutions and professional testing personnel, and the large number of samples submitted for testing, it is difficult to quickly obtain test results, and it is often necessary to wait in line for testing

[0006] (3) On-site rapid detection cannot be realized: Each cycle of traditional PCR includes three steps of denaturation, annealing and extension. Therefore, an instrument with precise temperature regulation must be used. It is difficult to apply outdoor or on-site detection, and must rely on professional Laboratories and professional experimenters, so on-site rapid detection of inspection materials cannot be realized

[0007] (4) For corrupt samples or old samples, the detection is relatively difficult. In order to obtain accurate results, multiple detections are required

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