A primer set, kit and detection method for rapid detection of human dys385 gene

A detection kit and primer set technology, which is applied in the field of forensic detection, can solve problems such as the limited number of detection personnel, the impossibility of on-site rapid detection of detection materials, and the difficulty of detection, so as to save material costs and time costs, and the results are intuitive and visualized , The effect of low detection environment requirements
CN110791557BActive Publication Date: 2022-06-28THE FIRST RES INST OF MIN OF PUBLIC SECURITY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST RES INST OF MIN OF PUBLIC SECURITY
Publication Date
2022-06-28

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Abstract

The invention discloses a primer set for rapidly detecting human DYS385 gene. Wherein, the primer set is composed of the following primers: primer F1 as shown in SEQ ID No.1; primer B1 as shown in SEQ ID No.2; primer F2-1 as shown in SEQ ID No.3; primer B2-1 As shown in SEQ ID No.4; Primer F2-2 as shown in SEQ ID No.5; Primer B2-2 as shown in SEQ ID No.6; Primer LOOP-F as shown in SEQ ID No.7; Primer LOOP -B is shown in SEQ ID No.8. The detection time required by the present invention is short, and the corresponding primers and kit can quickly detect the human DYS385 gene within 30 minutes to determine the sex of the human sample.
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Description

technical field

[0001] The invention relates to a detection kit and a method, in particular to a primer set, a kit and a detection method for rapid detection of human DYS385 gene, belonging to the field of forensic detection. Background technique

[0002] Humans have a total of 46 chromosomes, of which 22 are autosomes and the other two are sex chromosomes. Females have XX and males have XY. The DNA level identification of the human Y chromosome can be achieved by amplifying the unique genes of the human Y chromosome. The X chromosome cannot amplify the target fragment, but the Y chromosome can amplify the target fragment. Relying on this principle, the currently commonly used forensic DNA gender identification method is to use an identification kit to amplify the test material in a professional laboratory environment, and to detect the amplified product on a capillary electrophoresis apparatus. By amplifying the DYS38 locus, the female sample has no amplification peak, but...

Claims

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