Steroid hormone detection method

A steroid hormone and detection method technology, applied in the field of steroid hormone detection, can solve the problems of long detection time and many operation steps, and achieve the effect of short detection time and few operation steps.

Inactive Publication Date: 2020-02-21
杭州度安医学检验实验室有限公司
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Problems solved by technology

[0004] In order to solve the problems in the prior art that the sample needs to be pretreated step by step, there are

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Embodiment Construction

[0076] The present invention will be further described in detail below in conjunction with specific examples. In order to highlight the focus of the present invention, some conventional operations are omitted or simply described herein.

[0077] It should be understood that: although the present invention has been described in more detail through specific implementations, these descriptions are not intended to limit the patent scope of the present invention. Changes in form and details all belong to the protection scope of the present invention.

[0078] The samples of the methodological research experiment in this paper come from Hangzhou Du'an Medical Laboratory.

[0079] The instruments used in this paper include: Triple Quad 4500MD&Jasper HPLC triple quadrupole mass spectrometer (USA, AB SCIEX); medical high-speed refrigerated centrifuge (Hunan Xiangyi Laboratory Instrument Development Co., Ltd.); ultrapure water instrument (Canada Pallford Di Bioanalytical Instrument (S...

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Abstract

The invention discloses a steroid hormone detection method that comprises the following steps: adding an internal standard substance into a sample to be detected; carrying out a derivatization reaction; detecting an analyte by using chromatography tandem mass spectrometry; the analyte comprises a first steroid hormone and a second steroid hormone at the same time; the first steroid hormone is selected from at least one of the following steroid hormones: estradiol and estriol; and the second steroid hormone is selected from at least one of the following steroid hormones: dehydroepiandrosterone,dehydroepiandrosterone sulfate, testosterone, dihydrotestosterone, androstenedione, cortisol, cortisone, 11-deoxycortisol, 17alpha-hydroxyprogesterone, 17alpha-hydroxypregnenolone, aldosterone, cortisol, deoxycortisol, progesterone and pregnenolone. The detecting method has the advantages of few operation steps and short detection time, and can specifically, sensitively, accurately and quantitatively analyze the analyte substance.

Description

technical field [0001] The invention belongs to the technical field of hormone detection methods, in particular to a steroid hormone detection method. Background technique [0002] Steroid hormones such as cortisone and aldosterone can be quantified directly by chromatography-tandem mass spectrometry without the need for derivatization reactions. Chinese patent CN110187043A discloses "a method for simultaneously detecting 13 steroid hormones in serum", which can simultaneously detect pregnenolone, progesterone, 17-hydroxypregnenolone, 17α-hydroxyprogesterone, and 11α-hydroxyprogesterone Ketones, 21-deoxycortisol, 11-deoxycortisol, corticosterone, hydrocortisone, 11-deoxycortisol, cortisone, aldosterone, and estrone. However, the content of estradiol and estriol in organisms is relatively low, so they are not easy to be analyzed, and it is difficult to accurately quantify them in the detection. Therefore, testers usually derivatize estradiol and estriol before detection, so...

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 高飞徐可丰海云陈亮张志栋
Owner 杭州度安医学检验实验室有限公司
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