Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of dehydronitrosonisoldipine in preparing of drugs for sepsis prevention and treatment

A technology of nitrosinoxone and sepsis, applied in the field of medicine, can solve the problem that the effect of inhibiting pyroptosis has not been reported yet

Active Publication Date: 2020-02-25
NANJING UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This study shows that NTS has less toxic and side effects, but the effects of Guannisoldipine and NTS on inhibiting pyroptosis and preventing sepsis have not yet been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of dehydronitrosonisoldipine in preparing of drugs for sepsis prevention and treatment
  • Application of dehydronitrosonisoldipine in preparing of drugs for sepsis prevention and treatment
  • Application of dehydronitrosonisoldipine in preparing of drugs for sepsis prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: NTS inhibits endotoxin-induced pyroptosis of human monocyte cell line THP-1.

[0025] Experimental materials: NTS (MedChemExpress, Cat. No. HY-Z0816) was dissolved in dimethylsulfoxide (DMSO). Endotoxin Escherichia coli O111:B4 LPS (Sigma, Cat. No. LPS25) was dissolved using sterile water. The THP-1 cell line was provided by the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, and was cultured using RMPI 1640 culture medium supplemented with 10% fetal bovine serum and 1% penicillin / streptomycin. The Neon electroporator was purchased from Invitrogen. The model of optical microscope is Olympus IX71.

[0026] Experimental method: We added 50 μM NTS or an equal volume of DMSO to THP-1 cell culture medium and shaken; after 30 minutes, according to the method described in the literature (Zhao Y, Shi J, Shao F. Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis[J].Methods Mol Biol,2018,1714:131-148), using...

Embodiment 2

[0028] Example 2: NTS inhibits Nigerian toxin-induced pyroptosis of iBMDM cells in a mouse macrophage cell line.

[0029] Experimental materials: NTS (MedChemExpress, Cat. No. HY-Z0816), nigericin (Sigma, Cat. No. N7143) were dissolved in DMSO. The immortalized mouse-derived macrophage cell line (immortalized bonemarrow derived macrophage, iBMDM) was generously provided by Academician Shao Feng of the Beijing Institute of Life Sciences. It was cultured in RMPI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin / streptomycin. The model of optical microscope is Olympus IX71.

[0030] Experimental method: We added 50 μM NTS or an equal volume of DMSO to the cell culture medium of iBMDM and shaken; 30 minutes later, 10 μM nigericin was added to the culture medium to induce cell pyroptosis. After 1 hour, we observed and photographed cell morphology using a light microscope.

[0031] Experimental results: see attached figure 2 , panel A shows the negative con...

Embodiment 3

[0032] Example 3: NTS improves the survival rate of LPS sepsis mice and inhibits the expression of inflammatory factors

[0033] Experimental material: NTS (MedChemExpress, Cat.No.HY-Z0816) was dissolved in DMSO. Endotoxin Escherichia coli O111:B4 LPS (Sigma, Cat. No. LPS25) was dissolved using sterile water. The mice are C57 / BL6 strain, male, aged 6-8 weeks, weighing about 25 g, provided by the Model Animal Research Center (MARC) of Nanjing University. Mice were kept in a specific pathogen free (SPF) environment certified by the International Laboratory Animal Evaluation and Accreditation Committee, and all experiments were approved by the MARC Animal Care and Use Committee. The levels of interleukin (interleukin, IL)-1β, IL-6 and tumor necrosis factor-α (tumor necrosis factor-α, TNF-α) were detected by ELISA kits (Cat.No.MLB00C, Cat.No.M6000B, and Cat.No.M6000B, and Cat. .No.MTA00B, R&D Systems).

[0034] Experimental method: NTS was formulated with DMSO at a concentration ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicine, and relates to application of dehydronitrosonisoldipine (NTS) in preparing drugs for sepsis prevention and treatment, wherein the sepsis includes endotoxemia and multi-bacterial infectious sepsis. A particular mechanism involves the inhibition of pyroptosis by NTS. The drugs in the application refer to an intraperitoneal injection, and a dosage of the injection is within a range of 14.8 to 44.4 mg / kg ( body weight). In a septic mouse model, the drugs can inhibit the expression of plasma inflammatory factors, prolong the survival time of a mouse and raise the survival rate of the mouse.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the prevention and treatment effect of dehydronitrosonisoldipine (NTS) on sepsis; the specific mechanism involves NTS improving the inflammatory state of the body by inhibiting cell pyroptosis. Background technique [0002] Sepsis (sepsis) is a serious complication of surgical infection, and is the disease with the highest fatality rate in the intensive care unit; on average, one death will occur in every four patients with sepsis; there are about 31.5 million cases of sepsis in the world every year 5.3 million people died from sepsis. The pathological process of sepsis is extremely complex, and it has not been generally understood until the past three decades. The pathological process of sepsis can be divided into two stages, the early stage of inflammatory storm and the late stage of immunosuppression. The early inflammatory storm begins with the overreaction of the innate immune system...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4422A61P31/04A61P29/00A61P43/00
CPCA61K31/4422A61P31/04A61P29/00A61P43/00Y02A50/30
Inventor 高翔林兆宇王鼎玉陈芊月郑嘉烁
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com