7-hydroxymitragynine extraction method and traditional Chinese medicine composition containing 7-hydroxymitragynine

A technology of mitragynine and extraction method, applied in the field of medicine, can solve problems such as hidden safety hazards, poor physiotherapy effect, and difficult to wear, and achieve the effects of anti-fatigue muscle pain, high extraction rate and purity, and muscle pain relief.

Active Publication Date: 2020-02-25
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above products often have disadvantages such as poor physical therapy effect or difficult to wear, and sometimes even have potential safety hazards

Method used

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  • 7-hydroxymitragynine extraction method and traditional Chinese medicine composition containing 7-hydroxymitragynine
  • 7-hydroxymitragynine extraction method and traditional Chinese medicine composition containing 7-hydroxymitragynine
  • 7-hydroxymitragynine extraction method and traditional Chinese medicine composition containing 7-hydroxymitragynine

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Embodiment 1

[0041] This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition comprising 7-OH-mitragynine, and a health care belt comprising a traditional Chinese medicine composition; wherein,

[0042] The extraction method of 7-OH-mitragynine comprises the following steps:

[0043] (a) crush the dried kratom leaves, add chloroform according to the solid-liquid ratio of 1:10, adjust the pH value to 6.11 with a 10% acetic acid solution by mass fraction, perform ultrasonic extraction for 45 min at room temperature, and centrifuge to take the supernatant, This process was repeated 3 times, and the 3 supernatants were concentrated under reduced pressure to obtain the first concentrated solution; wherein, the ultrasonic power was 200W;

[0044] (b) Add an equal volume of petroleum ether to the first concentrated solution, extract at 50° C., take the lower layer liquid after standing, repeat this process 3 times, combine the lower layer liquid 3...

Embodiment 2

[0052] This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition comprising 7-OH-mitragynine, and a health care belt comprising a traditional Chinese medicine composition; wherein,

[0053] The extraction method of 7-OH-mitragynine comprises the following steps:

[0054] (a) Grind the dried kratom leaves, add chloroform according to the solid-liquid ratio of 1:12, adjust the pH value to 6.11 with 10% acetic acid solution by mass fraction, ultrasonically extract for 1.5 h at room temperature, and centrifuge to take the supernatant , this process was repeated 3 times, and the supernatant was concentrated under reduced pressure for 3 times to obtain the first concentrated solution; wherein, the ultrasonic power was 200W;

[0055] (b) Add an equal volume of petroleum ether to the first concentrated solution, extract at 40°C, and take the lower layer liquid after standing. This process is repeated 3 times, and the lower layer liqu...

Embodiment 3

[0061] This embodiment provides a method for extracting 7-OH-mitragynine, a traditional Chinese medicine composition comprising 7-OH-mitragynine, and a health care belt comprising a traditional Chinese medicine composition; wherein,

[0062] The extraction method of 7-OH-mitragynine comprises the following steps:

[0063] (a) crush the dried kratom leaves, add chloroform according to the solid-liquid ratio of 1:15, adjust the pH value to 6.28 with 10% acetic acid solution with a mass fraction, ultrasonically extract for 30 min at room temperature, and centrifuge to take the supernatant, This process was repeated 3 times, and the 3 supernatants were concentrated under reduced pressure to obtain the first concentrated solution; wherein, the ultrasonic power was 400W;

[0064] (b) Add an equal volume of petroleum ether to the first concentrated solution, extract at 45°C, and take the lower layer liquid after standing. This process is repeated 3 times, and the lower layer liquid i...

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Abstract

A 7-hydroxymitragynine extraction method and a traditional Chinese medicine composition containing 7-hydroxymitragynine are disclosed. The method includes the following steps: (1) crushing dried kratom leaves, adding an extractant into the crushed leaves, performing ultrasonic extraction at normal temperature, and performing centrifugation and vacuum concentration to obtain a first concentrate; (2) adding an extractant into the first concentrate for extraction and performing vacuum concentration to obtain a second concentrate; (3) subjecting the second concentrate to separation by silica gel column chromatography, and performing gradient elution with an eluent to obtain the 7-hydroxymitragynine. The method is simple to operate and fast, the extraction rate of the obtained 7-hydroxymitragynine is 0.24%, the obtained 7-hydroxymitragynine has purity of greater than 90% so that the extraction rate and the purity are high. The traditional Chinese medicine composition containing 7-hydroxymitragynine has the compound effect of refreshing the brain, resisting fatigue and alleviating muscle pain.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for extracting 7-OH-mitragynine and a traditional Chinese medicine composition containing 7-OH-mitragynine. Background technique [0002] Kratom is a medicinal plant of the genus Kratom that grows in Southeast Asian countries such as Thailand and Malaysia. It is also found in Yunnan at an altitude of 1,000 meters in my country. The leaves have been used as far back as the late 1800's to treat muscle pain, diarrhea, coughs, and to reduce the intake of costly opioids and to treat the withdrawal effects of opiate addiction. In addition, Malaysians and Thais also consume this plant to enhance their endurance for hard work under the hot sun. The main active ingredient of Kratom is indole alkaloids, of which mitragynine accounts for more than 66% of the total alkaloids, and 7-OH-mitragynine is a new alkaloid isolated in recent decades. Studies have found that it has stronge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D459/00A61K31/475A61K36/254A61K36/77A61K36/534A61P25/26A61P21/00A61P29/00A61P39/00A61F7/02A61M35/00
CPCC07D459/00
Inventor 徐丛玥乐凯
Owner JIANGHAN UNIVERSITY
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