Maize small-grain mutant and application thereof

A technology for mutants and small grains, applied in the fields of genetic engineering and molecular biology, can solve the problems of shrinking and shrinking of mutant grains, functional differences of NRT1.5, etc.

Active Publication Date: 2020-02-28
MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report in Arabidopsis that the NRT1.5 mutation will affect the grain development
However, NRT1.5 is mainly expressed in the kernels of maize, and the mutant kernels are smaller and shrunken, which may be due to the functional differences of NRT1.5 caused by the differences between monocots and dicots.

Method used

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  • Maize small-grain mutant and application thereof
  • Maize small-grain mutant and application thereof
  • Maize small-grain mutant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Phenotype Analysis of Maize Small Kernel Mutant mn2-m1

[0029] The small-grain mutant mn2-m1 of maize was discovered by the applicant in a pile of eliminated breeding materials. After testing, the mutant spontaneously showed small kernels from the early stage of pollination, and the kernels appeared small and shrunken after maturity (such as figure 1 shown), and its embryonic development is also affected (as figure 2 shown), which was significantly smaller than that of the wild type.

[0030] The experiments shown in Table 1 showed that the length, width, thickness and 100-grain weight of the mutant mn2-m1 grains were all reduced compared with the wild type, and its seedling development was also affected (such as image 3 shown).

[0031] Figure 1-3 and the a / A, b / B, c / C, d / D involved in Table 1 are from the F2 population of mn2-m1 and maize inbred lines B73, Chang 7-2, Zheng 58 and Qi 319, respectively.

[0032] Table 1 Analysis of grain traits

[003...

Embodiment 2

[0036] Example 2 Genetic Analysis

[0037] F1S was obtained by crossing the small corn mutant mn2-m1 with common maize inbred lines B73, Chang 7-2, Zheng 58 and Qi 319, and F2S was obtained by selfing F1S. Genetic analysis was carried out on the F2 segregation population, and the results showed that mn2-m1 The granule phenotype of is controlled by a recessive single gene (see Table 2 below).

[0038] Table 2 Phenotype segregation analysis of five parents and their F1 and F2 populations

[0039]

[0040] WT, wild type; χ2 (0.05,1) =3.84.

Embodiment 3

[0041] Example 3 Allelic Mutant

[0042] In 2016, mn2-m2 was obtained from the eliminated breeding material, and its phenotype was similar to mn2-m1. In order to verify whether they come from the same site mutation, we hybridized mn2-m1 / B73 with mn2-m2, and found that their F1 showed a 1:1 segregation, which indicated that both mn2-m1 and mn2-m2 came from the MN2-M mutation ( See Table 3).

[0043] Table 3 F hybridization between mn2-m1 / B73 and mn2-m2 1 Grain Phenotype Isolation

[0044] hybrid ear Wild type mutant Separation ratio chi-square value P-value spike-1 154 139 1.11 0.77 0.3

[0045] chi-square value (0.05,1) =3.84.

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Abstract

The invention belongs to the technical field of gene engineering and molecular biology and particularly relates to a maize small-grain mutant and application thereof. Maize grain mutant genes mn2-m1 and mn2-m2 are provided, Zm00001d019294 is obtained through map-based cloning to serve as a candidate gene for the grain mutant genes mn2-m1 and mn2-m2, a maize grain development regulation function ofZm00001d019294 is definite, a maize grain size affecting and regulation function of the gene is verified, transgenic crops in yield higher than that of wild type plants can be obtained, and accordingly theoretical bases can be provided for application of the gene to the field of breeding large-grain transgenic commercial crops. The mutant is capable of providing the theoretical bases and gene sources for breeding of new crop varieties and remarkably significant in genetic improvement breeding of maize germplasm resources.

Description

technical field [0001] The invention belongs to the technical fields of genetic engineering and molecular biology, and in particular relates to a small corn grain mutant and its application. Background technique [0002] Maize is an important food crop and a typical C4 model plant in my country, and plays an important role in the research of food production and functional genomics of monocots. With the rapid growth of global corn demand, the status of corn in the national economy has become increasingly prominent. The demand for corn in the world is increasing year by year, and the corn consumption structure has undergone fundamental changes, that is, from the main food crop that solves food and clothing, it has gradually developed into a diversified pattern of livestock feed, industrial raw materials, table non-staple food, and energy crops. Especially in recent years, the fields of renewable energy and deep processing have endowed corn with new connotations, which has led...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 关海英汪黎明刘铁山董永斌鲁守平何春梅刘春晓董瑞刘强王娟
Owner MAIZE RES INST SHANDONG ACAD OF AGRI SCI
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