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Preparation method of amino acid assisted SERS activity test paper

An amino acid and activity technology, applied in measurement devices, instruments, material analysis by optical means, etc., can solve the problems of low activity, poor uniformity of SRES substrate, low sensitivity, etc., achieving simple raw materials, poor uniformity improvement, and simple preparation method. Effect

Active Publication Date: 2020-03-24
JILIN UNIV
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Problems solved by technology

[0005] The present invention proposes a preparation method of an amino acid-assisted SERS activity test paper, first soaking filter paper of a standard size with L-arginine (Arg), and then loading silver nanoparticles on the filter paper by the method of in situ reduction, compared with the control group Compared with the SERS active test papers obtained with different silver loadings, the Raman spectrum enhancement of the 4-mercaptobenzoic acid (4-MBA) probe is realized. The outstanding advantages of this method are that the raw materials are simple and low-cost, and the operation is safe. Preparation method Simple and reproducible, the prepared test paper is easy to carry, flexible, and has high SERS activity, which is expected to improve the disadvantages of poor uniformity, low sensitivity, and low activity of existing SRES substrates

Method used

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  • Preparation method of amino acid assisted SERS activity test paper
  • Preparation method of amino acid assisted SERS activity test paper
  • Preparation method of amino acid assisted SERS activity test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The experimental conditions and parameters are as follows:

[0020] 1) Weigh 0.0523g L-arginine (C 6 h 14 N 4 o 2 ) was dissolved in 30mL deionized water to obtain a 0.01mol / L L-arginine aqueous solution. Weigh 0.1274g silver nitrate (AgNO 3 ) was dissolved in 30mL deionized water to obtain a 0.025mol / L silver nitrate aqueous solution. and make up 10 -4 mol / L 4-MBA ethanol aqueous solution.

[0021] 2) Take clean and dry medium-speed quantitative filter paper, and cut it into several square filter paper pieces with a size of 3cm×3cm for later use.

[0022] 3) Put the 3cm×3cm filter paper in a centrifuge tube filled with 30mL amino acid aqueous solution, shake it up and down slightly to make it fully soaked, let it soak for 30min, then take out the filter paper, wash it with deionized water and dry it at room temperature for later use.

[0023] 4) Put all the filter paper pieces in step 3) in a centrifuge tube filled with 30mL silver nitrate aqueous solution, sha...

Embodiment 2

[0028] The difference between this example and Example 1 is that in step 1) 30 mL of deionized water was taken as a blank control instead of the amino acid aqueous solution. Weigh 0.1274g silver nitrate (AgNO 3 ) was dissolved in 30mL deionized water to obtain a 0.025mol / L silver nitrate aqueous solution. and make up 10 -4 mol / L 4-MBA ethanol aqueous solution. Other steps and parameters are the same as in Embodiment 1.

[0029] According to the method cited in the above-mentioned invention, a kind of amino acid-assisted SERS activity test paper can be prepared, and its characteristics are as follows:

[0030] 1) SEM and energy spectrum analysis were carried out on the prepared amino acid-assisted SERS activity test paper. figure 2 Is the energy spectrum (EDS) figure of the SERS activity test paper prepared by the present invention in Examples 1 and 2. The results showed that in addition to the main components C and O of cellulose itself, there was also the presence of si...

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Abstract

The invention relates to a preparation method of an amino acid assisted SERS activity test paper. Generally speaking, the method comprises the steps of firstly using the L-arginine for treating a filter paper with a standard size; loading the silver nanoparticles on the filter paper through an in-situ reduction method to obtain the SERS activity test papers with different sliver load capacities, and realizing the Raman enhancement of the SERS activity test papers on a 4-mercaptobenzoic acid (4-MBA) probe. Result shows that compared with a control group, the test paper pretreated by L-arginineis large in silver load capacity, uniform in distribution, small in particle size, high in SERS activity, good in uniformity and high in stability. As a Raman-enhanced substrate, the SERS activity test paper has the characteristics of being simple, convenient and rapid to prepare, being convenient to carry, being high in flexibility and high in activity, can be applied to the dye detection, waterquality monitoring, biological detection and the like, and has the wide application prospect.

Description

Technical field: [0001] The invention relates to a method for preparing an amino acid-assisted surface-enhanced Raman spectrum flexible test paper substrate. In a nutshell, the present invention is based on the Raman spectrum of the silver nanoparticle-enhanced probe molecule, using amino acids to increase the loading of the silver nanoparticle on the test paper, improve the distribution of the nanoparticle, and further improve the SERS when detecting substances. active. Background technique: [0002] Metal nanomaterials have unique surface plasmon resonance (SPR) properties, and have important applications in many fields such as chemical and biological sensing, disease diagnosis and treatment, and catalysis. Surface-enhanced Raman scattering (SERS) technology is the application of this property of metal nanoparticles. For decades, surface-enhanced Raman spectroscopy (SERS) has been increasingly studied in analytical chemistry at the point of sample origin as a possible sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 郑先亮李宁王欣王赞田惠博于显利
Owner JILIN UNIV
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