Detection method for preventing and treating ulceration canceration mechanism by spleen-tonifying, heat-clearing and blood-activating formula

A technology for clearing heat and promoting blood circulation and detection method, which can be used in measurement devices, biological tests, material inspection products, etc., and can solve the problem that the detection method has not been reported yet.

Pending Publication Date: 2020-03-31
广西中医药大学附属瑞康医院
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up, the existing problems in the prior art are: there is no report on the detection method of the anti-ulcer carcinogenesis of the prescription of Jianpi Qingre Huoxue in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method for preventing and treating ulceration canceration mechanism by spleen-tonifying, heat-clearing and blood-activating formula
  • Detection method for preventing and treating ulceration canceration mechanism by spleen-tonifying, heat-clearing and blood-activating formula
  • Detection method for preventing and treating ulceration canceration mechanism by spleen-tonifying, heat-clearing and blood-activating formula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1: Main progress, results and impact of research work.

[0101] (1) Main research content.

[0102] The research of the present invention is mainly carried out from two angles of in vivo and in vitro experiments: firstly, the DMH / DSS composite method is used to prepare ulceration-related cancer models in vivo, and the Jianpi Qingre Huoxue formula is applied to the above models, and the PI3K-Akt / mTOR The pathway mediates the changes of related indicators in β-catenin phosphorylation, and explores the mechanism of Jianpi Qingre Huoxue prescription in preventing and treating ulcer-related cancer. Based on the experimental method of serum pharmacology in vitro, the quality standard of the drug-containing serum of Jianpi Qingre Huoxue Formula was established, and the drug-containing serum was applied to SW480 cells, and the changes of related indicators in β-catenin phosphorylation mediated by the PI3K-Akt / mTOR pathway were discussed. Mechanism of Jianpi Qingre Huox...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of canceration mechanism verification, and discloses a detection method for preventing and treating a ulceration canceration mechanism by a spleen-tonifying, heat-clearing and blood-activating formula. The spleen-tonifying, heat-clearing and blood-activating formula is used for preventing and treating ulceration canceration (UCAC) and is related to mediation of a PI3K-Akt / mTOR pathway to cooperate with a Wnt pathway to regulate and control P-beta-catenin; in-vivo experiments adopt a modern biotechnology to detect colon mucosa morphology, an ultrastructure, PI3K-Akt / mTOR pathway related indexes and p-beta-catenin intranuclear transposition; and cell proliferation, apoptosis, cycle, indexes in a PI3K-Akt / mTOR pathway and beta-catenin intranucleartransposition are detected by virtue of an in-vitro experiment. The invention proves that the spleen-tonifying, heat-clearing and blood-activating formula for preventing and treating UCAC is possiblyrelated to the mediation of the PI3K-Akt / mTOR pathway cooperated with the Wnt pathway for regulating and controlling P-beta-catenin, and a new thought is provided for preventing and treating UCAC by traditional Chinese medicines.

Description

technical field [0001] The invention belongs to the technical field of carcinogenesis mechanism verification, and in particular relates to a method for detecting the carcinogenesis mechanism of ulcers prevented by the prescription of invigorating spleen, clearing heat and promoting blood circulation. Background technique [0002] At present, the closest prior art: Ulcerative colitis (ulcerative colitis, UC) is a non-controllable inflammatory disease that is prone to canceration. In recent years, the incidence rate in China has been increasing year by year. K-ras gene mutation, abnormal activation of p53, epidermal growth factor (EGF) and its receptor family (epidermal growth factor receptor, EGFR) etc. colitis associated carcinogenesis, UCAC) plays an important role in the occurrence. However, in the prior art, there is no report on the detection method of the mechanism of preventing and treating ulceration and carcinogenesis with the formula of strengthening the spleen, cl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/574G01N33/48
CPCG01N33/68G01N33/57419G01N33/4833
Inventor 张涛陈思羽黄会云陈远能郑东林黄适
Owner 广西中医药大学附属瑞康医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap